Structural Characterization and Immunoenhancing Effects of a Polysaccharide from the Soft Coral Lobophytum sarcophytoides

• Published in: Marine biotechnology (New York, N.Y.) N.Y.), 2022-03, Vol.24 (1), p.203-215
• Main Authors: Cao, Xueqin, Zhang, Qian, Zhu, Yanglu, Li, Siju, Cai, Ying, Li, Pei, Liu, Deliang, Leng, Yun, Ye, Simin, Xu, Zengmei, Li, Hao, Shen, Baochun, Liao, Qiongfeng, Liu, Lan, Xie, Zhiyong
• Format: Article
• Language: English
• Subjects: Animals; Anthozoa - metabolism; Biomedical and Life Sciences; Biotechnology; Cell proliferation; Corals; Engineering; Food industry; Freshwater & Marine Ecology; Gene expression; IL-1β; Immunostimulants; Industry; Interleukin 6; Life Sciences; Lobophytum; Mice; Microbiology; Molecular weight; NF-kappa B - metabolism; NF-κB protein; Original Article; Phagocytosis; Pharmaceutical sciences; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Proliferation; Proteins; RAW 264.7 Cells; Reagents; Saccharides; Signal Transduction; Structural analysis; TLR4 protein; Toll-like receptors; Tumor necrosis factor-α; Zoology
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-022-10099-y

Previous studies on the soft coral Lobophytum sarcophytoides ( Lobophytum sp.) are mainly about small molecules, and there has been no systematic research on polysaccharides. In the study, a novel polysaccharide (LCPs-1-A) with immunoenhancing functions was successfully extracted and purified from the soft coral Lobophytum sp. After preliminary analysis, our data indicated that LCPs-1-A was composed of glucose and had a molecular weight of 4.90 × 10 6  Da. Moreover, our findings showed that LCPs-1-A could promote the proliferation and phagocytosis of RAW264.7 cells, stimulate the production of NO and ROS, and increase the mRNA expression of IL-1β, IL-6, and TNF-α, which indicated that LCPs-1-A had a good immunoenhancing activity. Through further studies, we found that LCPs-1-A might play an immunoenhancing role through the TLR4/NF-κB signaling pathway. Therefore, our results demonstrated that LCPs-1-A might be a natural immunostimulant for use in medical and food industries.