Study on the levels of N-nitrosamine compounds and untargeted metabolomics in patients with colorectal cancer

• Published in: Analytical and bioanalytical chemistry 2022-05, Vol.414 (11), p.3483-3496
• Main Authors: Luo, Zhi, Wang, Hetao, Lin, Simin, Liao, Lili, Cai, Lishan, Zhang, Xiaoxu, Tan, Yangying, Shen, Mei
• Format: Article
• Language: English
• Subjects: Abnormalities; Adenine; Adenosine monophosphate; AMP; Analytical Chemistry; Biochemistry; Biomarkers; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography, Liquid; Cluster analysis; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms; Discriminant analysis; Drug therapy; Encyclopedias; Female; Food Science; Genomes; Guanine; Humans; Hypoxanthine; Laboratory Medicine; Lysophosphatidic acid; Male; Metabolic pathways; Metabolism; Metabolites; Metabolomics; Monitoring/Environmental Analysis; N-Nitrosodimethylamine; N-Nitrosopiperidine; N-Nitrosopyrrolidine; Negative ions; Nitrosamine; Nitrosamines - analysis; Research Paper; Tandem Mass Spectrometry; Xanthine; Xanthines
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-022-03969-w

Plasma samples were collected from 34 patients with advanced CRC and 92 healthy persons (control group), and the levels of 9 VNAs were measured using GC–MS. Untargeted metabolomics analysis was performed using LC–MS/MS. Partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis were used to determine differential metabolites between the 2 groups. Receiver operating characteristic (ROC) curve analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis were performed on the differential metabolites. It turned out that the detection rates of N-nitrosodimethylamine (NDMA) and N-nitrosopyrrolidine (NPYR) in patients with CRC were higher than in the control group ( P