Caldalkalibacillus salinus sp. nov., isolated from a salt lake in Xinjiang, northwest China

• Published in: Archives of microbiology 2022-03, Vol.204 (3), p.179-179, Article 179
• Main Authors: Ouyang, Ting-Hao, Peng, Man, Zhang, Zhen, Chunyu, Wei-Xun, Wang, Li-Ming, Zhou, En-Min, Gao, Xu-Hong, Tang, Shu-Kun
• Format: Article
• Language: English
• Subjects: Bacillaceae; Bacterial Typing Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Cell Biology; China; DNA, Bacterial - genetics; Ecology; Fatty acids; Fatty Acids - analysis; Gene sequencing; Genomes; Lakes - microbiology; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; Nucleic Acid Hybridization; Nucleotide sequence; Original Paper; Peptidoglycans; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - analysis; Phylogenetics; Phylogeny; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Salt lakes; Sediment samplers; Sequence Analysis, DNA; Sodium chloride
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-02789-x

A novel Gram-stain-negative, aerobic, motile and rod-shaped bacterium, designated as strain YIM B00319 T , was isolated from a sediment sample obtained from Wuzunbulake salt Lake in Xinjiang Uygur Autonomous Region, northwest China. Phylogenetic analysis based on 16S rRNA gene sequences along with the whole genome showed that strain YIM B00319 T belongs to the family Bacillaceae and was most closely related to Bacillus horti K13 T and Caldalkalibacillus mannanilyticus JCM 10596 T , with sequence similarities of 95.7% and 94.6%, respectively. The genome of strain YIM B00319 T was 3.77 Mbp with a DNA G + C content of 43%. Strain YIM B00319 T grew at 15–45 ℃, pH 7.0–9.5 and with 3–11% (w/v) NaCl. The major respiratory quinone of strain YIM B00319 T was MK-7, and the major fatty acids (> 10%) were iso-C 15:0 , anteiso-C 15:0 , and summed feature 3 (C 16:1 ω 6 c and/or C 16:1 ω 7 c ). The main polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and diphosphatidylglycerol (DPG). The cell-wall peptidoglycan contained meso -diaminopimelic acid. On the basis of the phenotypic, chemotaxonomic, genomic, and phylogenetic information, strain YIM B00319 T represents a novel species of the genus Caldalkalibacillus , for which the name Caldalkalibacillus salinus sp. nov. is proposed. The type strain is YIM B00319 T (= CGMCC 1.18750 T  = NBRC 115338 T ).