Gryllus bimaculatus De Geer hydrolysates alleviate lipid accumulation, inflammation, and endoplasmic reticulum stress in palmitic acid-treated human hepatoma G2 cells

Nonalcoholic fatty liver disease (NAFLD) is one of the most common hepatic diseases closely intertwined with saturated fatty acids intake. Therefore, various studies are being conducted to find natural substances to prevent either the onset or progression of NAFLD. According to traditional medicinal...

Full description

Saved in:
Bibliographic Details
Published in:Journal of ethnopharmacology 2022-06, Vol.291, p.115117-115117, Article 115117
Main Authors: Kim, Nayeon, Jung, Sunyoon, Lee, Eunjung, Jo, Eun-Byeol, Yoon, Seongjun, Jeong, Yoonhwa
Format: Article
Language:English
Subjects:
Endoplasmic reticulum stress
Gryllus bimaculatus De Geer
Human hepatoma G2 cells
Inflammation
Lipid accumulation
Palmitic acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nonalcoholic fatty liver disease (NAFLD) is one of the most common hepatic diseases closely intertwined with saturated fatty acids intake. Therefore, various studies are being conducted to find natural substances to prevent either the onset or progression of NAFLD. According to traditional medicinal literature, it has been reported that Gryllus bimaculatus De Geer (GB) has systemic detoxifying activity; however, the preventive effects of GB on NAFLD have not been elucidated to date. To evaluate the potential of GB as a material for the mitigation of NAFLD, we investigated the effects of GB hydrolysates on the hepatic lipid accumulation, inflammation, and endoplasmic reticulum (ER) stress in human hepatoma G2 (Hep G2) cells treated with palmitic acid (PA). Steamed and dried GB was defatted, pulverized, and then lyophilized following hydrolyzation using Neutrase® (GB-N) or Flavourzyme® (GB-F). Hep G2 cells were incubated with GB-N or GB-F at various concentrations (0, 0.25, 0.5, and 1 mg/mL) for 24 h, and then PA was treated for another 24 h. The GB-N and GB-F significantly prevented the PA-induced intracellular lipid accumulation in the human liver cells (p 
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2022.115117