Insights into the response mechanism of Litopenaeus vannamei exposed to cold stress during live transport combining untargeted metabolomics and biochemical assays

• Published in: Journal of thermal biology 2022-02, Vol.104, p.103200-103200, Article 103200
• Main Authors: Xu, Defeng, Zheng, Xiaoxian, Li, Caihong, Wu, Jiaxin, Sun, Lijun, Qin, Xiaoming, Fan, Xiuping
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Biochemical analysis; Cold; Cold-Shock Response; Glycolysis; Hepatopancreas; Hepatopancreas - metabolism; Ions; Liquid chromatography; Litopenaeus vannamei; Mass spectroscopy; Metabolomics; Metabolomics - methods; Penaeidae - metabolism; Penaeidae - physiology; Physiology; Ultrastructure
• ISSN: 0306-4565; 1879-0992
• DOI: 10.1016/j.jtherbio.2022.103200

Unfavorable conditions severely affect the survival quality of Litopenaeus vannamei (L. vannamei) during live transport and the molecular response mechanism needs to be clarified. In this study, metabolomics combined with conventional assay on physiological and histopathological responses were applied to deepen the understanding of L. vannamei to cold stress and provide. A solid foundation for regulation of transportation management. Physiological and biochemical analysis revealed the significant disturbance of glycolysis in hepatopancreas tissue. Furthermore, metabolomics based on the technique of ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry identified the significantly differential metabolites between acute cold exposure (AC) and normal control (NC) groups. Moreover, KEEG result indicated that the pathways of amino acid, glycerophospholipid, and nuclei acid metabolism and ABC transporters in hepatopancreas were significantly disturbed. Furthermore, histopathology and ultrastructure verified the impairment in hepatopancreas during cold stress. Overall, the concurrent use of metabolomics and biochemical assays was demonstrated to be sensitive and effective in providing new insights into the response mechanism of L. vannamei to cold stress. [Display omitted] •Metabolomics with physiology and ultrastructure elucidated adaptive mechanism.•Glycolysis disturbance was revealed in L. vannamei to cold stress.•Metabolomics revealed the significantly differential metabolites under cold stress.•Metabolism pathways were significantly altered under cold stress.•Histopathology and ultrastructure revealed the impairment in hepatopancreas.