Nanoencapsulated Lippia rotundifolia antimicrobial peptide: synthesis, characterization, antimicrobial activity, and cytotoxicity evaluations

Antimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The...

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Bibliographic Details
Published in:Archives of microbiology 2022-03, Vol.204 (3), p.184-184, Article 184
Main Authors: dos Santos, Elisandra M., Tavares, Letícia S., Fayer, Leonara, Brandão, Humberto M., Munk, Michele, Santos, Marcelo de O.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibiotics
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Antimicrobial Peptides
Atomic force microscopes
Atomic force microscopy
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Chitosan
Chitosan - pharmacology
Cytotoxicity
E coli
Ecology
Encapsulation
Escherichia coli
HEK293 Cells
Humans
Life Sciences
Lippia
Microbial Ecology
Microbiology
Minimum inhibitory concentration
Nanoparticles
Original Paper
Particle Size
Peptides
Stability
Staphylococcus epidermidis
Toxicity
Zeta potential
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Summary:Antimicrobial peptides (AMP) are promising novel antibiotics but exhibit low stability and can be toxic. The AMP encapsulation can be used to protect the drug and control its release rates. The Lr-AMP1f encapsulated into chitosan nanoparticle (NP) by ionic gelation method reached 90% efficiency. The results indicated that the hydrodynamic particle size of NPs increased from 196.1 ± 3.14 nm (free NP) to 228.1 ± 12.22 nm (nanoencapsulated Lr-AMP1f), while the atomic force microscope showed the spherical shape. The Zeta potential of the nanoencapsulated Lr-AMP1f was high (+ 35 mV). These AMP-loaded NPs exhibited stability for up to 21 days of storage. The minimum inhibitory concentration (MIC) of free Lr-AMP1f was 8 µg/mL for E. coli and S. epidermidis. However, the nanoencapsulated Lr-AMP1f produced a bacteriostatic effect against both bacteria at 8 µg/mL . The MIC of nanoencapsulated Lr-AMP1f was 16 µg/mL for E. coli and 32 for S. epidermidis . Nanoencapsulated Lr-AMP1f was nontoxic to HEK293 cells. Promisingly, chitosan NP can be used as a vehicle for the antibacterial application of new AMP (Lr-AMP1f).
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-022-02787-z