Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching

• Published in: Clinical oral investigations 2022-05, Vol.26 (5), p.4099-4108
• Main Authors: Ortecho-Zuta, Uxua, de Oliveira Duque, Carla Caroline, de Oliveira Ribeiro, Rafael Antonio, Leite, Maria Luísa, Soares, Diana Gabriela, Hebling, Josimeri, Briso, André Luiz Fraga, de Souza Costa, Carlos Alberto
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials; Biomaterials; Biomedical materials; Bleaching; Catalysts; Cattle; Cell culture; Coatings; Cytotoxicity; Dental Enamel; Dentin; Dentistry; Esthetics, Dental; Hydrogen Peroxide; Hypochlorous Acid; Medicine; Original Article; Oxidative stress; Tooth Bleaching; Tooth Bleaching Agents - toxicity
• ISSN: 1436-3771; 1432-6981; 1436-3771
• DOI: 10.1007/s00784-022-04379-z

Objectives Evaluate in vitro the esthetic efficacy and cytotoxicity of a bleaching gel containing 35% hydrogen peroxide (BG-35%H 2 O 2 ), applied for different time intervals, on enamel coated or not with polymeric biomaterials. Materials and methods Nanofiber scaffolds (NSc) and a primer catalyst (PrCa) were used to coat the bovine enamel/dentin discs before the application of BG-35%H 2 O 2 , according to the following groups: G1—negative control (NC, without treatment); G2, G3, and G4—BG-35%H 2 O 2 applied for 3 × 15, 2 × 15, and 15 min; G5, G6, and G7—BG-35%H 2 O 2 applied on enamel coated with NSc and PrCa for 3 × 15; 2 × 15, and 15 min, respectively. The culture medium with components of gel diffused through the discs was applied on MDPC-23 cells, which were evaluated regarding to viability (VB), integrity of the membrane (IM), and oxidative stress (OxS). The quantity of H 2 O 2 diffused and esthetic efficacy (Δ E /Δ WI ) of the dental tissues were also analyzed (ANOVA/Tukey; p   0.05). The lowest value of H 2 O 2 diffusion occurred in G4 and G7, where the cells exhibited the lowest OxS than G2 ( p