Tannin as a modulator of rumen microbial profile, apparent digestibility and ingestive behavior of lactating goats: A preliminary metagenomic view of goats adaptability to tannin

• Published in: Research in veterinary science 2022-07, Vol.145, p.159-168
• Main Authors: Silva de Sant'ana, Aline, Ribeiro Silva, Ana Paula, Oliveira do Nascimento, Sheyla Priscila, Araújo Moraes, André, Fonseca Nogueira, Joel, Moreira Bezerra, Flávia Caroline, Fraga da Costa, Camila, de Simoni Gouveia, João José, Veneroni Gouveia, Gisele, Torres de Souza Rodrigues, Rafael, Colombarolli Bonfa, Hugo, Ribeiro Menezes, Daniel
• Format: Article
• Language: English
• Subjects: Adaptability; Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animals; Behavior; Carbohydrates; Data collection; Diet; Diet - veterinary; Digestibility; Digestion; DNA sequencing; Evaluation; Experiments; Factorial design; Feces; Feeds; Female; Fermentation; Food intake; Goat farming; Goats; Goats - metabolism; Lactation; Metagenome; Metagenomics; Microbial responses; Microbiomes; Microbiota; Microorganisms; Naturalized goats; Nutrients; Phenolic compounds; Physiological adaptation; Relative abundance; Rumen; Rumen - metabolism; Sheep; Statistical methods; Tannins; Tannins - metabolism; Variance analysis; Veterinary medicine; Visual observation
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2022.02.002

The aim with this study was to use interdisciplinary techniques and visions in order to identify the modulating effect of tannins on adaptive factors in lactating goats with different genetic patterns, through the assessment of digestibility and nutrient intake, ingestive behavior and rumen metagenome. We used in this study 8 of the Repartida ecotype and 6 of the Canindé breed goats, on average five years old, distributed in a completely randomized design, in a 2 × 2 factorial design, with two genetic groups and two diets. The applied diets were: basal diet and inclusion of 5% commercial tannin extract in basal diet. Intake and apparent digestibility of nutrients were evaluated, based on the quantification of the offered feed and refusals, and bromatological evaluation of samples of the offered feed, refusals and feces. Behavioral data were collected in 24-h continuous visual observations. The ruminal fluid was collected and DNA extraction, sequencing, and evaluation of relative abundance of the rumen microbiome were performed. The data obtained were analyzed statistically, through analysis of variance with 5% significance and, when necessary, a comparison of means test was applied. In this preliminary findings was observed that the genetic group factor caused changes in the number of chews and the relative abundance of microorganisms (P = 0.0290 and P = 0.0051). The diet factor influenced digestibility, which better values were observed for the tannin diet (P = 0.0049), in addition, it promoted changes in the rumen microbiota, with a beneficial modulatory characteristic. The inclusion of 5% tannin extract from Acacia mearnsii modulates the rumen microbiome, improving the apparent digestibility of nutrients without affecting the feed intake of goats from the Repartida and Canindé genetic groups. •Tannin at 5% level in diet improves nutrients digestibility in goats.•There are differences in rumen microbiome of different genetic groups goats.•Tannin at 5% level in diet alters the abundance of several rumen bacteria.•Methanogenic archeas abundance are reduced in response to 5% tannin in diet.•Some ruminal bacteria species are resistant to condensed tannin inclusion.