Enhancement of Cryo-EM maps by a multiscale tubular filter

We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures a...

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Bibliographic Details
Published in:Optics express 2022-01, Vol.30 (3), p.4515-4527
Main Authors: Vargas, Javier, Gómez-Pedrero, José A, Quiroga, J Antonio, Alonso, José
Format: Article
Language:English
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Summary:We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures and to attenuate blob-like and plate-like structures. The approach, thus, introduces a priori information in the reconstructions to improve their interpretability and analysis at high-resolution. The proposed method has been tested with simulated and real cryo-EM maps including recent reconstructions of the SARS-CoV-2. Our results show that our methods can improve obtained reconstructions.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.444675