Improvement of spatial resolution in photoacoustic microscopy using transmissive adaptive optics with a low-frequency ultrasound transducer

Maintaining a high spatial resolution in photoacoustic microscopy (PAM) of deep tissues is difficult due to large aberration in an objective lens with high numerical aperture and photoacoustic wave attenuation. To address the issue, we integrate transmission-type adaptive optics (AO) in high-resolut...

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Bibliographic Details
Published in:Optics express 2022-01, Vol.30 (2), p.2933-2948
Main Authors: Notsuka, Yusuke, Kurihara, Makoto, Hashimoto, Nobuyuki, Harada, Yoshinori, Takahashi, Eiji, Yamaoka, Yoshihisa
Format: Article
Language:English
Subjects:
Animals
Blood Vessels - diagnostic imaging
Ear - blood supply
Equipment Design
Mice
Microscopy, Acoustic - instrumentation
Optics and Photonics
Photoacoustic Techniques - instrumentation
Transducers
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Summary:Maintaining a high spatial resolution in photoacoustic microscopy (PAM) of deep tissues is difficult due to large aberration in an objective lens with high numerical aperture and photoacoustic wave attenuation. To address the issue, we integrate transmission-type adaptive optics (AO) in high-resolution PAM with a low-frequency ultrasound transducer (UT), which increases the photoacoustic wave detection efficiency. AO improves lateral resolution and depth discrimination in PAM, even for low-frequency ultrasound waves by focusing a beam spot in deep tissues. Using the proposed PAM, we increased the lateral resolution and depth discrimination for blood vessels in mouse ears.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.446309