Nanoplastics enhance the toxic effects of titanium dioxide nanoparticle in freshwater algae Scenedesmus obliquus

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology 2022-06, Vol.256, p.109305-109305, Article 109305
• Main Authors: Das, Soupam, Thiagarajan, Vignesh, Chandrasekaran, N., Ravindran, Balasubramani, Mukherjee, Amitava
• Format: Article
• Language: English
• Subjects: Fluorescent nanoplastics; Fresh Water; Leachate; Microplastics; Nanoparticles - toxicity; nTiO2; Oxidative stress; Scenedesmus; Scenedesmus obliquus; Titanium - chemistry; Titanium - toxicity; Toxicity; Water Pollutants, Chemical - toxicity
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2022.109305

The increased usage of titanium dioxide nanoparticles (nTiO2) in consumer products has led to their prevalence in freshwater systems. Nanoplastics, a secondary pollutant, can significantly influence the toxic effects of nTiO2 in freshwater organisms. The present study investigates the role of fluorescent nanoplastics (FNPs) in modifying the harmful effects of P25 nTiO2 in freshwater algae Scenedesmus obliquus. Three different concentrations of nTiO2, 0.025, 0.25, and 2.5 mg/L, were mixed with 1 mg/L of the FNPs to perform the mixture toxicity experiments. The presence of the FNPs in the mixture increased the toxicity of nTiO2 significantly. A significant increment in the oxidative stress parameters like total ROS, superoxide (O2∎−), and hydroxyl radical generation was observed for the mixture of nTiO2 with the FNPs in comparison with their individual counterparts. The lipid peroxidation, and the antioxidant enzyme activities in the algal cells correlated well with the reactive species generation results. The treatments with the binary mixture resulted in notable decrease in the esterase activity in the algal cells. The mixture toxicity results were further validated with Abbott's independent action model. Additionally, optical microscopic analysis and FTIR analysis were performed to study the morphological and surface chemical changes in the algae. This study demonstrated that the FNPs played a key role in enhancing the toxicity of nTiO2 in freshwater algae. [Display omitted] •Heteroaggregation of nTiO2 and fluorescent nanoplastics in the test medium•nTiO2 and fluorescent polystyrene nanoplastics decrease the viability of S. obliquus.•nTiO2 and fluorescent polystyrene nanoplastics induce oxidative stress in algae.•Decrease in photosynthetic yield and esterase activity was observed.•nTiO2 and fluorescent polystyrene nanoplastics altered algal morphology.