Study of osmoadaptation mechanisms of halophilic Halomonas alkaliphila XH26 under salt stress by transcriptome and ectoine analysis

• Published in: Extremophiles : life under extreme conditions 2022-04, Vol.26 (1), p.14-14, Article 14
• Main Authors: Zhang, Tiantian, Zhang, Xin, Li, Yongzhen, Yang, Ning, Qiao, Lijuan, Miao, Zengqiang, Xing, Jiangwa, Zhu, Derui
• Format: Article
• Language: English
• Subjects: ABC transporter; Abiotic stress; Amino Acids, Diamino - metabolism; Assembly; Bacteria; Biochemistry; Biogenesis; Biomedical and Life Sciences; Biomedical materials; Biotechnology; Cell survival; Cytochromes; Ectoine; Flagella; Genes; Glycine; Glycine (amino acid); Glycine betaine; Halomonas; Halomonas - genetics; Halomonas - metabolism; Halophilic bacteria; Imports; Iron; Life Sciences; Microbial Ecology; Microbiological strains; Microbiology; Monosaccharides; Oligosaccharides; Original Paper; Phosphates; Phosphonates; Putrescine; Salinity; Salinity effects; Salt lakes; Salt Stress; Sodium chloride; Strain; Survival; Synthesis; Transcription; Transcriptome; Transcriptomes; Uptake
• ISSN: 1431-0651; 1433-4909
• DOI: 10.1007/s00792-022-01256-1

Halophilic bacteria such as the genus Halomonas are promising candidates in diverse industrial, agricultural and biomedical applications. Here, we successfully isolated a halophilic Halomonas alkaliphila strain XH26 from Xiaochaidan Salt Lake, and studied its osmoadaptation strategies using transcriptome and ectoine analysis. Divergent mechanisms were involved in osmoadaptation at different salinities in H. alkaliphila XH26. At moderate salinity (6% NaCl), increased transcriptions of ABC transporters related to iron (III), phosphate, phosphonate, monosaccharide and oligosaccharide import were observed. At high salinity (15% NaCl), transcriptions of flagellum assembly and cell motility were significantly inhibited. The transcriptional levels of ABC transporter genes related to iron (III) and iron 3+ -hydroxamate import, glycine betaine and putrescine uptake, and cytochrome biogenesis and assembly were significantly up-regulated. Ectoine synthesis and accumulation was significantly increased under salt stress, and the increased transcriptional expressions of ectoine synthesis genes ectB and ectC may play a key role in high salinity induced osmoadaptation. At extreme high salinity (18% NaCl), 5-hydroxyectoine and ectoine worked together to maintain cell survival. Together these results give valuable insights into the osmoadaptation mechanisms of H. alkaliphila XH26, and provide useful information for further engineering this specific strain for increased ectoine synthesis and related applications.