Molecular characterization, expression, and function of Vitellogenin genes in Phytoseiulus persimilis

• Published in: Experimental & applied acarology 2022-03, Vol.86 (3), p.343-356
• Main Authors: Zhenhui, Wang, Qi, Cai, Shuo, Yan, Shuoyu, Yang, Qin, Lu, Endong, Wang, Bo, Zhang, Jiale, Lv, Xuenong, Xu
• Format: Article
• Language: English
• Subjects: Adults; Animal Ecology; Animal Genetics and Genomics; Animal Systematics/Taxonomy/Biogeography; Animals; Biomedical and Life Sciences; Double-stranded RNA; Eggs; Embryogenesis; Embryonic growth stage; Embryos; Entomology; Female; Females; Gametocytes; Gene expression; Genes; Genomes; Life Sciences; Lipids; Male; Mites; Molecular weight; Peptides; Phytoseiulus persimilis; Proteins; Reproduction; Vitellogenin; Vitellogenins - genetics; Vitellogenins - metabolism
• ISSN: 0168-8162; 1572-9702; 1572-9702
• DOI: 10.1007/s10493-022-00698-8

Vitellogenin (Vg) is an important factor that impacts oocyte maturation, egg formation and embryonic development in Arthropoda. Two orthologs of Vg gene were obtained from the genome of Phytoseiulus persimilis and termed as PpVg1 and PpVg2 . Both orthologs belong to the large lipid transfer protein superfamily. The expression of PpVg1 and PpVg2 was low in immatures and male adults, and increased rapidly in female adults after mating, and reached a peak before the first egg was laid (168×  and 20.5×  the level in virgin females, respectively). When PpVg1 and PpVg2 were interfered with dsRNA, the relative expression decreased by 81.0 and 30.9%, respectively, and 7.8 and 31.4% interfered individuals died within 24 h. Among surviving individuals, ca. 51.1 and 44.8% are infertile. Factors that might be related to expression of Vg genes are also discussed.