EMP80 mediates the C‐to‐U editing of nad7 and atp4 and interacts with ZmDYW2 in maize mitochondria

• Published in: The New phytologist 2022-05, Vol.234 (4), p.1237-1248
• Main Authors: Zhao, Jiao, Cao, Shi‐Kai, Li, Xiu‐Lan, Liu, Rui, Sun, Feng, Jiang, Rui‐Cheng, Xu, Chunhui, Tan, Bao‐Cai
• Format: Article
• Language: English
• Subjects: Amino acids; Arginine; Corn; Editing; Embryogenesis; Embryonic growth stage; EMP80; Endosperm; Genes; maize (Zea mays); Mitochondria; Mitochondria - metabolism; Mitochondrial Proteins - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - metabolism; PPR protein; Proteins; Ribonucleic acid; RNA; RNA editing; Seeds - genetics; Zea mays; Zea mays - metabolism; ZmDYW2; ZmNUWA
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.18067

Summary RNA C‐to‐U editing is important to the expression and function of organellar genes in plants. Although several families of proteins have been identified to participate in this process, the underlying mechanism is not fully understood. Here we report the function of EMP80 in the C‐to‐U editing at the nad7‐769 and atp4‐118 sites, and the potential recruitment of ZmDYW2 as a trans deaminase in maize (Zea mays) mitochondria. Loss of EMP80 function arrests embryogenesis and endosperm development in maize. EMP80 is a PPR‐E+ protein localised to mitochondria. An absence of EMP80 abolishes the C‐to‐U RNA editing at nad7‐769 and atp4‐118 sites, resulting in a cysteine‐to‐arginine (Cys→Arg) change in Nad7 and Atp4 in the emp80 mutant. The amino acid change consequently reduces the assembly of complexes I and V, leading to an accumulation of the F1 subcomplex of complex V. EMP80 was found to interact with atypical DYW‐type PPR protein ZmDYW2, which interacts with ZmNUWA. Co‐expression of ZmNUWA enhances the interaction between EMP80 and ZmDYW2, suggesting that EMP80 potentially recruits ZmDYW2 as a trans deaminase through protein–protein interaction, and ZmNUWA may function as an enhancer of this interaction.