CC chemokine 1 protein from Cromileptes altivelis (CaCC1) promotes antimicrobial immune defense

• Published in: Fish & shellfish immunology 2022-04, Vol.123, p.102-112
• Main Authors: Yang, Xiaoyu, Wu, Ying, Zhang, Panpan, Chen, Guisen, Cao, Zhenjie, Ao, Jingqun, Sun, Yun, Zhou, Yongcan
• Format: Article
• Language: English
• Subjects: Animals; Anti-Infective Agents; Antimicrobial immunity; Bass; CC chemokine 1; Chemokines - genetics; Chemokines, CC - genetics; Chromileptes altivelis; Fish Diseases; Fish Proteins - chemistry; Gene Expression Regulation; Mammals - metabolism; Migration; Phagocytosis; Phylogeny
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2022.02.032

Chemokines are a family of small signaling proteins that are secreted by various cells. In addition to their roles in immune surveillance, localization of antigen, and lymphocyte trafficking for the maintenance of homeostasis, chemokines also function in induce immune cell migration under pathological conditions. In the present study, a novel CC chemokine gene (CaCC1) from humpback grouper (Cromileptes altivelis) was cloned and characterized. CaCC1 comprised a 435 bp open reading frame encoding 144 amino acid residues. The putative molecular weight of CaCC1 protein was 15 kDa CaCC1 contains four characteristic cysteines that are conserved in other known CC chemokines. CaCC1 also shares 11.64%–90.28% identity with other teleost and mammal CC chemokines. Phylogenetic analysis revealed that CaCC1 is most closely related to Epinephelus coioides EcCC1, both of which are in a fish-specific CC chemokine clade. CaCC1 was constitutively expressed in all examined C. altivelis tissues, with high expression levels in skin, heart, liver, and intestine. Vibrio harveyi stimulation up-regulated CaCC1 expression levels in liver, spleen, and head-kidney. Functional analyses revealed that the recombinant protein (rCaCC1) could induce the migration of head-kidney lymphocytes from C. altivelis. Moreover, rCaCC1 significantly enhanced phagocytosis in head-kidney macrophages from C. altivelis. In addition, rCaCC1 exhibited antimicrobial activities against Staphylococcus aureus, Edwardsiella tarda, and V. harveyi. In vivo, CaCC1 overexpression improved bacterial clearance in V. harveyi infected fish. Conversely, CaCC1 knockdown resulted in a significant decrease of bacterial clearance. These results demonstrate the important roles that CaCC1 plays in homeostasis and in inflammatory response to bacterial infection. •New CC chemokine (CaCC1) was cloned from humpback grouper (Cromileptes altivelis).•Infection up-regulated CaCC1 expression in liver, spleen, and head-kidney.•rCaCC1 induced head-kidney lymphocyte migration and macrophage phagocytosis.•CaCC1 exhibited antibacterial activities, and overexpression improved clearance.