Carrageenan oligomers and salicylic acid act in tandem to escalate artemisinin production by suppressing arsenic uptake and oxidative stress in Artemisia annua (sweet wormwood) cultivated in high arsenic soil

• Published in: Environmental science and pollution research international 2021-08, Vol.28 (31), p.42706-42721
• Main Authors: Naeem, Muhammad, Aftab, Tariq, Ansari, Abid Ali, Khan, Mohammad Masroor Akhtar
• Format: Article
• Language: English
• Subjects: Antioxidants; Aquatic Pollution; Arsenic; Artemisia annua; Artemisinin; ascorbate peroxidase; Ascorbic acid; Atmospheric Protection/Air Quality Control/Air Pollution; Carrageenan; Catalase; Crop yield; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Foliage; Growth regulators; Horticultural crops; horticulture; irradiation; L-Ascorbate peroxidase; leaves; Malaria; Oligomers; Oxidative stress; Parameters; Peroxidase; Plant growth; Research Article; Salicylic acid; soil; Soils; stress tolerance; Superoxide dismutase; Toxicity; Vector-borne diseases; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-13241-w

The present study is aimed to elucidate the effects of concomitant application of irradiated carrageenan (IC) oligomers and salicylic acid (SA) on Artemisia annua L. varieties, viz. “CIM-Arogya” (tolerant) and “Jeevan Raksha” (sensitive) exposed to arsenic (As) stress. Artemisia annua has been known for its sesqui-terpene molecule artemisinin, which is useful in curing malaria. The two compounds, IC and SA, have been established as effective plant growth-promoting molecules for several agricultural and horticultural crops. To test the stress tolerance providing efficacy of IC and SA, the characterization of various physiological and biochemical parameters, growth as well as yield attributes was done in the present experiment. A. annua plants were given various treatments viz. (i) Control (0) (ii) 45 mg As kg -1 of soil (iii) 80 mg L -1 IC+45 mg As kg -1 of soil (iv) 10 -6 M SA+45 mg As kg -1 of soil (vi) 45 mg As kg -1 soil+80 mg L -1 IC+10 -6 M SA. Plants of A. annua suffered from prominent injuries due to oxidative stress generated by As. At 90 and 120 days after planting (DAP), As toxicity was manifested in reduction of most of the studied growth parameters. However, antioxidant activities such as catalase (CAT), peroxidase (POX), superoxide dismutase (SOD), and ascorbate peroxidase (APX) were enhanced in As-stressed conditions and their activities were further enhanced in IC+SA-treated plants. Application of As significantly produced the highest artemisinin content and yield in “CIM-Arogya” over “Jeevan Raksha.” Noticeably, the selected plant growth regulators (PGRs) (IC and SA) applied individually through foliage were found efficient, though, the concomitant effect of PGRs was much pronounced compared to their alone application in countering the toxicity of As. The interactive action of PGRs escalated the overall production (yield) of artemisinin (58.7% and 53.8%) in tolerant and sensitive varieties of A. annua in the presence of soil As. Conclusively, the concomitant application of IC and SA proved much effective and successful over their individual use in exploring the overall development of A. annua subjected to As stress.