In vitro elimination of EL4 cancer cells from spermatogonia stem cells by miRNA-143- and 206-loaded folic acid-conjugated PLGA nanoparticles

MiRNA's-143 and -206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- and 206-loaded poly(lactic- -glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. The therapy was conducted in six groups: treatment...

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Published in:Nanomedicine (London, England) England), 2022-04, Vol.17 (8), p.531-545
Main Authors: Shams, Azar, Shabani, Ronak, Asgari, Hamidreza, Karimi, Mahdi, Najafi, Mohammad, Asghari-Jafarabadi, Mohammad, Razavi, Seyed Mohsen, Miri, Seyed Rouhollah, Abbasi, Mehdi, Mohammadi, Amirhossein, Koruji, Morteza
Format: Article
Language:English
Subjects:
Folic Acid
Humans
Lactic Acid
Male
MicroRNAs - genetics
Nanoparticles
Neoplasms
Polyethyleneimine
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Spermatogonia
Stem Cells
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Summary:MiRNA's-143 and -206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- and 206-loaded poly(lactic- -glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. The therapy was conducted in six groups: treatment with both miRNAs simultaneously (mixed miRNAs), miRNA-206 treatment, miRNA-143 treatment, blank PLGA, blank polyethylenimine (PEI) and complex PEI-miRNAs. In terms of viability, in mixed miRNAs no synergistic effect was observed on EL4 cell elimination. However, in the single miRNA-206 group, a stronger apoptotic effect was observed than the mixed miRNAs group and single miRNA-143 group alone. MiRNAs' apoptotic induction effects in cancer cells were found to be remarkable.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2021-0210