Pseudohalioglobus sediminis sp. nov., isolated from coastal sediment

• Published in: Archives of microbiology 2022-04, Vol.204 (4), p.207-207, Article 207
• Main Authors: Zhang, Ya-Ning, Zhang, Tian-Shu, Chen, Xu-Yang, Gong, Ya, Du, Zong-Jun
• Format: Article
• Language: English
• Subjects: Archives & records; Bacterial Typing Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Catalase; Cell Biology; Coastal zone; Coasts; DNA, Bacterial - genetics; Ecology; Fatty acids; Genes; Genomes; Life Sciences; Lipids; Marine sediments; Microbial Ecology; Microbiology; Morphology; Motility; New species; Original Paper; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - chemistry; Phylogenetics; Phylogeny; Physiology; Quinones; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seawater - microbiology; Sediments; Sequence Analysis, DNA; Sodium chloride
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-02816-x

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, designated NY5 T , was isolated from marine sediment collected from coastal area in Weihai, China (122°07′ 38.80′′ E, 37°33′ 57.60′′ N). Cells of strain NY5 T were 0.6–0.7 μm width and 1.9–2.0 μm length, catalase-positive and oxidase-positive. Growth of NY5 T was observed at 25–37 °C (optimum, 28 °C) and pH 6.5–9.5 (optimum, pH 7.5–8.0) and in the presence of 0.5–7.0% (w/v) NaCl (optimum, 2.0%). The isoprenoid quinone was Q-8 and the predominant fatty acids were summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ), summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ) and C 17:1 ω 8 c . Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. The DNA G+C content of strain NY5 T was 60.1%. Strain NY5 T showed the highest 16S rRNA gene sequence similarity (98.2%) with Pseudohalioglobus lutimaris followed by Parahaliea aestuarii (96.9%), Parahaliea maris (96.7%), Parahaliea mediterranea (95.9%), and Halioglobus japonicus (94.9%). Given these phenotypic and chemotaxonomic properties and phylogenetic analyses, strain NY5 T was considered to represent a novel species of the genus Pseudohalioglobus , for which the name Pseudohalioglobus sediminis sp. nov. is proposed. The type strain is NY5 T (=KCTC 72416 T =MCCC 1H00401 T ).