DNAzyme-controlled plasmonic coupling for SERS-based determination of Salmonella typhimurium using hybridization chain reaction self-assembled G-quadruplex

• Published in: Mikrochimica acta (1966) 2022-04, Vol.189 (4), p.140-140, Article 140
• Main Authors: Xu, Wanqing, Leng, Xueqi, Jin, Tong, Liu, Su, Wang, Yu, Huang, Jiadong
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biosensing Techniques - methods; Cellular signal transduction; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Coupling; DNA, Catalytic - metabolism; Environmental monitoring; Environmental testing; Food; G-Quadruplexes; Hybridization; Limit of Detection; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Oxidation; Plasmonics; Safety and security measures; Salmonella; Salmonella typhimurium - genetics; Salmonella typhimurium - metabolism; Self-assembly; Signal transduction; Thiols
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-021-05154-9

A facile and rapid SERS strategy for S. typhimurium detection based on hybridization chain reaction (HCR) self-assembled G-quadruplex DNAzyme (GQH DNAzyme)-controlled plasmonic coupling was developed. GQH DNAzyme is introduced as a biocatalyst to catalyze the oxidation of l -cysteines to cysteines (thiols to disulfides) to assist SERS signal transduction. This is the first time that the self-assembled split GQH DNAzyme-controlled plasmonic coupling is integrated with SERS sensing. The results reveal the proposed SERS strategy can quantify S. typhimurium with a wide linear range (5 to 10 5  cfu mL −1 ) and a low detection limit (4 cfu mL −1 ; n  = 5, mean ± standard deviation) and RSD of 7%. The method exhibited preeminent detection performance in spiked samples with recoveries of 93.1–117%. The proposed strategy has great potential for being a versatile SERS platform for detecting a wide spectrum of analytes by replacing them with the corresponding recognition elements. Therefore, this study not only creates a practical platform for pathogenic bacteria identification and related food safety testing and environmental monitoring, but also provides a new paradigm for building SERS sensor. Graphical abstract A facile and rapid SERS strategy for S. Typhimurium detection based on hybridization chain reaction (HCR) self-assembled G-quadruplex DNAzyme-controlled plasmonic coupling