Controlled PAH-mediated method with enhanced optical properties for simple, stable immunochromatographic assays

Despite their potential for signal amplification in immunochromatographic assays (ICAs) with Au nanoparticles (AuNPs) as probes, metal growth methods are of limited practical applicability given their complex non-specificity and lack of robust growth schemes. Here, we propose a novel method of polya...

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Published in:Biosensors & bioelectronics 2022-06, Vol.206, p.114150-114150, Article 114150
Main Authors: Shao, Yanna, Xu, Wenxing, Zheng, Yin, Wang, Juan, Xie, Jihang, Zhu, Zhenjun, Xiang, Xinran, Ye, Qinghua, Zhang, Youxiong, Xue, Liang, Gu, Bing, Chen, Jierong, Zhang, Jumei, Wu, Qingping, Ding, Yu
Format: Article
Language:English
Subjects:
Au nanoparticles
Biosensing Techniques
Escherichia coli O157
Escherichia coli O157:H7
Food Microbiology
Gold - chemistry
Immunoassay
Immunochromatographic assay
Metal growth
Metal Nanoparticles - chemistry
Polyallylamine hydrochloride
Reproducibility of Results
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Summary:Despite their potential for signal amplification in immunochromatographic assays (ICAs) with Au nanoparticles (AuNPs) as probes, metal growth methods are of limited practical applicability given their complex non-specificity and lack of robust growth schemes. Here, we propose a novel method of polyallylamine hydrochloride (PAH)-mediated metal growth for the detection of Escherichia coli O157:H7 by AuNP-ICA. The developed method relies on the highly controlled growth of Cu shells on the AuNP core and allows one to achieve highly enhanced colorimetric signals by controlling PAH as the growth framework. The introduction of PAH eliminates the non-specific adsorption of Cu ions on the nitrocellulose membrane and thus provides maximized and effective signal-to-noise ratios to achieve a detection limit of 9.8 CFU/mL for E. coli O157:H7. Moreover, the newly developed detection method exhibits good reproducibility (coefficient of variation
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2022.114150