Alkaloid profiling of Italian alpine herbs using high resolution mass spectrometry (Orbitrap-MS)

Alpine pastures have not yet been extensively studied with regard to the presence of alkaloids in herbaceous plants. In this work the alkaloid profiles were characterised from a selection of 62 herbs collected from alpine pastures in north-eastern Italy. High-performance liquid chromatography couple...

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Bibliographic Details
Published in:Natural product research 2023-07, Vol.37 (15), p.2583-2590
Main Authors: Nardin, Tiziana, Larcher, Roberto, Barnaba, Chiara, Bertoldi, Daniela, Pasut, Davide, Romanzin, Alberto, Piasentier, Edi
Format: Article
Language:English
Subjects:
Alkaloids
Alkaloids - analysis
Chromatography, High Pressure Liquid - methods
Discriminant analysis
Herbs
High performance liquid chromatography
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Pasture
Plant Extracts - chemistry
Plants
Quadrupoles
Tandem Mass Spectrometry - methods
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Summary:Alpine pastures have not yet been extensively studied with regard to the presence of alkaloids in herbaceous plants. In this work the alkaloid profiles were characterised from a selection of 62 herbs collected from alpine pastures in north-eastern Italy. High-performance liquid chromatography coupled to a hybrid quadrupole-orbitrap mass spectrometer was used to evaluate the presence of 41 different alkaloids and quantify them using a targeted approach. Provisionally, 118 alkaloids were identified, including both free and glycosylated forms, making use of a homemade database and a suspect screening approach. rucifoline, gramine, heliotrine, lycopsamine, seneciphylline, and veratramine were quantified with concentrations ranging from 6 to about 100 µg kg in 6 plants. Herbaceous plants belonging to the most well-represented plant families (Poaceae, 9 species; Asteraceae, 7; Lamiaceae, 6) showed distinct and characteristic alkaloid profiles and were correctly reclassified with an average accuracy of 85% (Partial Least Squares - Discriminant Analysis).
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2022.2050908