Identification and quantification of phytochemicals of Chamaecostus cuspidatus (Nees & Mart.) C.D.Specht & D.W.Stev and Cheilocostus speciosus (J. Koenig) C.D.Specht by LC-MS techniques and their in - vitro anti-adipogenic screening

(Nees & Mart.) C.D.Specht & D.W.Stev and (J.Koenig) C.D.Specht contain bioactive compounds that possess many pharmacological activities including antidiabetic and hypolipidemic. These plants are used to treat diabetes by herbal healers. Considering the traditional use of and , the present st...

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Bibliographic Details
Published in:Natural product research 2023-07, Vol.37 (14), p.2461-2465
Main Authors: Shukla, Vijaya, Bajpai, Vikas, Singh, Pratibha, Rai, Prashant, Khandelwal, Nilesh, Gaikwad, Anil N, Singh, Bikarma, Kumar, Brijesh
Format: Article
Language:English
Subjects:
Adipogenesis
Bioactive compounds
Biological activity
Campsurus cuspidatus
Diabetes mellitus
Guidelines
Leaves
Liquid chromatography
Phytochemicals
Qualitative analysis
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Summary:(Nees & Mart.) C.D.Specht & D.W.Stev and (J.Koenig) C.D.Specht contain bioactive compounds that possess many pharmacological activities including antidiabetic and hypolipidemic. These plants are used to treat diabetes by herbal healers. Considering the traditional use of and , the present study is designed to perform qualitative and quantitative analysis as well as anti-adipogenesis against 3T3-L1 cells to ensure efficacy. A total of thirty-eight compounds were identified using HPLC-QTOF-MS/MS. Quantification of ten bioactive compounds among identified compounds was performed by UPLC-QqQ -MS/MS. The quantification method was validated according to ICH guidelines (International conference on harmonization guidelines). Quantification of bioactive compounds of different organs of and showed remarkable differences in the content. Microscopic and ORO absorbance confirmed the antiadipogenic potential of leaves (L-02), roots (R-02) of and leaves of (L-01) in 3T3-L1 cells.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2022.2050225