Escherichia coli ST131 isolated from urological patients can acquire plasmid‐mediated extended spectrum β‐lactamase from other bacteria with high frequency

Objectives To investigate the prevalence of the clonal group Escherichia coli ST131 in urologic patients, and to clarify the mechanisms underlying the high prevalence of the antimicrobial resistant genes in ST131. Methods We used 65 Escherichia coli strains collected from the Department of Urology,...

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Bibliographic Details
Published in:International journal of urology 2022-06, Vol.29 (6), p.587-594
Main Authors: Kondo, Tsubasa, Sakamoto, Kei, Morinaga, Yoshitomo, Miyata, Yasuyoshi, Yanagihara, Katsunori, Sakai, Hideki
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
antimicrobial resistant
beta-Lactamases - genetics
Conjugation
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli Infections - drug therapy
Escherichia coli Infections - epidemiology
Escherichia coli Infections - microbiology
Humans
Levofloxacin
Mice
Multilocus sequence typing
Patients
Plasmids - genetics
Quinolones - pharmacology
ST131 strains
Strains (organisms)
urological patients
Vancomycin
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Summary:Objectives To investigate the prevalence of the clonal group Escherichia coli ST131 in urologic patients, and to clarify the mechanisms underlying the high prevalence of the antimicrobial resistant genes in ST131. Methods We used 65 Escherichia coli strains collected from the Department of Urology, Nagasaki University Hospital, between January 2018 and December 2018. All of them underwent multilocus sequence typing and were analyzed for genes associated with quinolone resistance and extended‐spectrum β‐lactamases. To compare ST131 and non‐ST131 strains, bacterial conjugation experiments and intestinal colonization evaluations were performed. Results ST131 was the most dominant among all the strains, along with levofloxacin resistant strains, and extended‐spectrum β‐lactamases positive strains (32%, 63%, and 73%, respectively). 12 out of 15 extended‐spectrum β‐lactamases‐producing Escherichia coli strains harbored CTX‐M‐9. In particular, all extended‐spectrum β‐lactamases‐producing ST131 strains possessed CTX‐M‐9. The proportions of ST131 strains with or without quinolone resistance‐determining region mutations were significantly higher and lower, respectively, than that of non‐ST131 strains (P = 0.0002 and P 
ISSN:0919-8172
1442-2042
DOI:10.1111/iju.14845