Advances in chemical and biological characterization of triterpenoid saponins from Anagallis arvensis L. using UHPLC-MS/MS and cell-based assays

• Published in: Natural product research 2023-07, Vol.37 (15), p.2596-2601
• Main Authors: Pastoriza, Ana Cristina, Sgariglia, Melina Araceli, Soberón, José Rodolfo, Sampietro, Diego Alejandro
• Format: Article
• Language: English
• Subjects: Anagallis arvensis; Chromatography, High Pressure Liquid; Cytotoxicity; Humans; Lymphocytes; Molecular Structure; NMR; Nuclear magnetic resonance; Oleanolic acid; Saponins; Saponins - chemistry; Saponins - pharmacology; Tandem Mass Spectrometry; Toxicity testing; Triterpenes - chemistry; Triterpenes - pharmacology
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2053122

A fraction enriched in triterpenoid saponins (F4) from L. was chemically characterized by UHPLC-ESI-QTOF-MS/MS and NMR analyses. The results proposed the presence of nine monodesmosidic saponins derived from oleanolic acid, including two reported for the first time for this species, : 3-O-{β-D-glucopyranosyl (1→4)-[β-D-xylopyranosyl (1→2)]-β-D-glucopyranosyl (1→4)-[β-D-glucopyranosyl (1→2)]-α-L-arabinopyranosyl}-13β, 28-epoxy- 22 acetyl- 28 methoxy-16α, 24-oleananediol and : 3-O-{β-D-glucopyranosyl (1→4)-β-D-glucopyranosyl (1→4)-[β-D-xylopyranosyl (1→2)]-β-D-glucopyranosyl (1→4)-[β-D-glucopyranosyl (1→2)]-α-L-arabinopyranosyl}-13β, 28-epoxy- 16α, 30-oleananediol. Furthermore, haemolytic activity was determined by dot-blot autography, and cytotoxicity on human lymphocyte cultures was analysed according to metabolic activity (MTT assay) and membrane integrity (Trypan blue exclusion test). F4 showed mild cytotoxicity (%V > 50% at 100 μg/mL) on human lymphocytes under conditions of activation or not by LPS; moreover, showed haemolytic activity between 50 and 500 μg. This work contributed to phytochemical knowledge of the triterpenoid saponins from , and its cytotoxic effects on normal human cells.