Complete Depletion of Primordial Germ Cells Results in Masculinization of Monopterus albus, a Protogynous Hermaphroditic Fish

• Published in: Marine biotechnology (New York, N.Y.) N.Y.), 2022-04, Vol.24 (2), p.320-334
• Main Authors: Hou, Mingxi, Feng, Ke, Luo, Hongrui, Jiang, Yinjun, Xu, Wen, Li, Yongming, Song, Yanlong, Chen, Ji, Tao, Binbin, Zhu, Zuoyan, Hu, Wei
• Format: Article
• Language: English
• Subjects: Animals; Aquaculture; Biomedical and Life Sciences; Catadromous fishes; Depletion; Differentiation; Eels; Eels - genetics; Eels - metabolism; Embryogenesis; Embryonic growth stage; Engineering; Female; Females; Fish; Freshwater & Marine Ecology; Freshwater fishes; Gene expression; Genes; Germ Cells; Gonads; Hermaphrodites; Hermaphroditism; Leydig cells; Life Sciences; Male; Male sterility; Marine fishes; Masculinization; Microbiology; Monopterus albus; Original Article; Ovaries; Rice fields; Sertoli cells; Sex; Sex Determination Processes - genetics; Sex differentiation; Sex Differentiation - genetics; Smegmamorpha; Zebrafish; Zoology
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-022-10106-2

Primordial germ cells (PGCs) play an important role in sexual fate determination and gonadal development in gonochoristic fish, such as zebrafish and medaka. However, little is known about the function of PGCs in hermaphroditic fish. Rice field eel ( Monopterus albus ), a protogynous hermaphroditic fish, is an economically valuable aquaculture species. We eliminated PGCs in rice field eels during embryogenesis via morpholino-mediated knockdown dead end ( dnd ). The PGCs-depleted gonads developed into testis-like structures with Sertoli cells and Leydig cells. The gene expression pattern of 15-month-old PGCs-depleted gonads showed that male-biased genes, dmrt1 , sox9a , gsdf , and amh , were significantly higher than that of the WT, whereas female-biased genes, foxl2 and cyp19a1a , were significantly decreased. These results indicate that PGCs are essential for ovarian differentiation in rice field eel, and PGCs-depleted gonads develop into sterile males without undergoing the female and intersex stages. Our study is the first to identify the role of PGCs in sex differentiation in rice field eel, a protogynous hermaphrodite teleost. And it is of great significance in rice field eel for discovering the underlying mechanism of sex differentiation and establishing sex control technology.