The dissimilarity of antibiotic and quorum sensing inhibitor on activated sludge nitrification system: Microbial communities and antibiotic resistance genes

• Published in: Bioresource technology 2022-05, Vol.351, p.127016-127016, Article 127016
• Main Authors: Gao, Yu-Xi, Li, Xing, Fan, Xiao-Yan, Zhao, Jun-Ru, Zhang, Zhong-Xing
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibiotic resistance genes; Archaea - genetics; Bacteria - metabolism; Bacterial and archaeal communities; Combined pollution; Drug Resistance, Microbial - genetics; Genes, Bacterial - genetics; Microbiota; Nitrification; Potential hosts; Quorum Sensing - genetics; Quorum sensing inhibitor; Sewage - microbiology
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2022.127016

[Display omitted] •Compare with AZM, nitrification was more inhibited by QSI.•AZM and QSI had more inhibitions on bacteria and archaea, respectively.•Functions differ little in bacteria/archaea and AZM had more impact on metabolism.•AZM had main impact on nitrifying bacteria and specific DNB were enriched.•Bacteria were main hosts for most ARGs and archaea carried multiple ARGs. Effects of antibiotics (azithromycin, AZM, 1–40 mg/L) and quorum sensing inhibitor (QSI, 2(5H)-furanone, 1–40 mg/L) combined pollution with environmental concentration of copper on bacterial/archaeal community and antibiotic resistance genes (ARGs) in activated sludge system were explored. QSI inhibited nitrification more obviously than AZM. AZM and QSI were synergistic inhibitions on bacterial diversity, and AZM inhibited bacterial compositions more than QSI. While, QSI had more impacts on archaeal diversity/compositions. Less interactions among bacteria and archaea communities with Aquimonas as keystone genus. Functional differences in bacteria/archaea communities were little, and AZM had more effects on metabolism. AZM mainly affected nitrifying bacteria (Candidatus Nitrospira nitrificans and Nitrosomonas). Specific denitrifying bacteria were enriched by AZM (Brevundimonas, 1.76–31.69%) and QSI (Comamonas, 0.61–9.61%), respectively. AZM enriched ARGs more easily than QSI and they were antagonistic to proliferation of ARGs. Bacteria were main hosts of ARGs (macrolide-lincosamide-streptogramin B, other/efflux, etc.) and archaea (Methanosphaerula, Methanolobus) carried multiple ARGs.