Surface plasmon resonance aptasensor for soluble ICAM-1 protein in blood samples

Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an ap...

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Bibliographic Details
Published in:Analyst (London) 2022-04, Vol.147 (8), p.1663-1668
Main Authors: Dursun, Ali Dogan, Dogan, Soner, Kavruk, Murat, Busra Tasbasi, B, Sudagidan, Mert, Deniz Yilmaz, M, Yilmaz, Bayram, Ozalp, Veli C, Tuna, Bilge G
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide
Atherosclerosis
Biomarkers
Biosensing Techniques
Biosensors
Blood
Gold
Humans
Intercellular Adhesion Molecule-1
Limit of Detection
Magnetic pull
Nanoparticles
Proteins
Surface Plasmon Resonance
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Summary:Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml −1 , which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles. An aptamer sequence for soluble ICAM-1 was selected by the SELEX procedure. The ICAM-1 aptamer was used to develop a magnetic separation from blood samples by silica shell nanoparticles and subsequent real-time detection by SPR biosensing.
ISSN:0003-2654
1364-5528
DOI:10.1039/d1an02332b