Enrichment of boron element in follicular fluid and its potential effect on the immune function

• Published in: Environmental pollution (1987) 2022-07, Vol.304, p.119147-119147, Article 119147
• Main Authors: Zhang, Guohuan, Wang, Anni, Zhuang, Lili, Wang, Xikai, Song, Ziyi, Liang, Rong, Ren, Mengyuan, Long, Manman, Jia, Xiaoqian, Li, Zhiwen, Su, Shu, Wang, Jiahao, Zhang, Nan, Shen, Guofeng, Wang, Bin
• Format: Article
• Language: English
• Subjects: Blood–follicle barrier; Boron; Boron - metabolism; Female; Fertilization in Vitro - methods; Follicular Fluid; Graphic abstract; Humans; Immunity; Immunoglobulin; In vitro fertilization and embryo transfer; Male; Oocytes - metabolism; Trace Elements - metabolism
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2022.119147

The blood–follicle barrier (BFB) between the blood and follicular fluid (FF) can maintain the microenvironment balance of oocyte. Boron, an exogenous environmental trace element, has been found to possibly play an important role in oocyte maturation. This study aimed to examine the distribution characteristics of boron across the BFB and find the potential effect of boron on FF microenvironment. We analyzed the concentration of boron in paired FF and serum collected from 168 women undergoing in vitro fertilization and embryo transfer in Beijing City and Shandong Province, China. To explore the potential health impact of boron enrichment in oocyte maturation, a global proteomics analysis was conducted to tentatively correlate the protein levels with the boron enrichment. Interestingly, the results showed that the concentration of boron in FF (34.5 ng/mL) was significantly higher than that in serum (22.0 ng/mL), with a median concentration ratio of 1.52. Likewise, the concentrations of boron in FF and serum were positively correlated (r = 0.446), suggesting that boron concentration in serum can represent its concentration in follicular fluid to a large extent.. This is the first time to observe the enrichment of boron in the FF to our knowledge. It is interesting to observe a total of 13 proteins, which mainly belong to immunoglobulin class, were positively correlated with boron concentration in FF. We concluded that boron, as one environmental trace element, was enriched in FF from blood validated by two area in north china, which may be involved in an increased level of immune processes of immunoglobulins. [Display omitted] •The boron concentration in follicular fluid is higher than that in serum.•The boron concentration in follicular and serum are positively correlated.•Boron is associated with increased immunoglobulins in follicular fluid.•Boron may contribute to maintaining the immune status in the follicular environment. Boron can enrich in female follicular fluid and has potential effects on immune processes induced by immunoglobulins.