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A simplified fluorescent lateral flow assay for melamine based on aggregation induced emission of gold nanoclusters
[Display omitted] •A novel LFA was designed based on the AIE of AuNCs for the detection of melamine.•Molecular docking analysis verifies melamine has the highest affinity toward ATT.•The sensor could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min. A novel lateral flow assay (LFA...
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Published in: | Food chemistry 2022-08, Vol.385, p.132670-132670, Article 132670 |
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creator | Yue, Xin Pan, Qiuli Zhou, Jie Ren, Hongxin Peng, Chifang Wang, Zhouping Zhang, Yi |
description | [Display omitted]
•A novel LFA was designed based on the AIE of AuNCs for the detection of melamine.•Molecular docking analysis verifies melamine has the highest affinity toward ATT.•The sensor could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min.
A novel lateral flow assay (LFA) was developed based on the aggregation induced emission (AIE) of gold nanoclusters (AuNCs) for the detection of melamine. We compared the interaction between melamine and AuNCs prepared with 6-Aza-2-thiothymine (ATT) and its four structural analogs as ligands and found that the ATT-AuNCs showed the best AIE induced by melamine. Then the ATT-AuNCs were directly immobilized on the test line of LFA strips as reporters. The sensing platform exhibited a linear response to melamine from 1 to 100 μM (R2 = 0.997) with a detection limit of 217 nM. Moreover, this LFA strip could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min. The recoveries of 91.6–101.5% were attained in milk and baby formula samples. This strip inherited the superior characteristics of low background interference of aggregation-induced fluorescence recognition and LFA technology, thus effectively avoiding the use of antibodies, aptamers, and other biological elements. |
doi_str_mv | 10.1016/j.foodchem.2022.132670 |
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•A novel LFA was designed based on the AIE of AuNCs for the detection of melamine.•Molecular docking analysis verifies melamine has the highest affinity toward ATT.•The sensor could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min.
A novel lateral flow assay (LFA) was developed based on the aggregation induced emission (AIE) of gold nanoclusters (AuNCs) for the detection of melamine. We compared the interaction between melamine and AuNCs prepared with 6-Aza-2-thiothymine (ATT) and its four structural analogs as ligands and found that the ATT-AuNCs showed the best AIE induced by melamine. Then the ATT-AuNCs were directly immobilized on the test line of LFA strips as reporters. The sensing platform exhibited a linear response to melamine from 1 to 100 μM (R2 = 0.997) with a detection limit of 217 nM. Moreover, this LFA strip could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min. The recoveries of 91.6–101.5% were attained in milk and baby formula samples. This strip inherited the superior characteristics of low background interference of aggregation-induced fluorescence recognition and LFA technology, thus effectively avoiding the use of antibodies, aptamers, and other biological elements.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2022.132670</identifier><identifier>PMID: 35318181</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aggregation induced emission ; Animals ; detection limit ; fluorescence ; Fluorescent Dyes - chemistry ; food chemistry ; Gold - chemistry ; Gold nanocluster ; Lateral flow assay ; ligands ; Limit of Detection ; Melamine ; Metal Nanoparticles - chemistry ; milk ; Milk - chemistry ; nanogold ; oligonucleotides ; Spectrometry, Fluorescence ; Triazines</subject><ispartof>Food chemistry, 2022-08, Vol.385, p.132670-132670, Article 132670</ispartof><rights>2022 Elsevier Ltd</rights><rights>Copyright © 2022 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-d4408c383762b865e241a0f413ea4c0d5b0b118170e8dcefd5a8a71ab8a927e53</citedby><cites>FETCH-LOGICAL-c401t-d4408c383762b865e241a0f413ea4c0d5b0b118170e8dcefd5a8a71ab8a927e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35318181$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yue, Xin</creatorcontrib><creatorcontrib>Pan, Qiuli</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Ren, Hongxin</creatorcontrib><creatorcontrib>Peng, Chifang</creatorcontrib><creatorcontrib>Wang, Zhouping</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><title>A simplified fluorescent lateral flow assay for melamine based on aggregation induced emission of gold nanoclusters</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>[Display omitted]
•A novel LFA was designed based on the AIE of AuNCs for the detection of melamine.•Molecular docking analysis verifies melamine has the highest affinity toward ATT.•The sensor could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min.
A novel lateral flow assay (LFA) was developed based on the aggregation induced emission (AIE) of gold nanoclusters (AuNCs) for the detection of melamine. We compared the interaction between melamine and AuNCs prepared with 6-Aza-2-thiothymine (ATT) and its four structural analogs as ligands and found that the ATT-AuNCs showed the best AIE induced by melamine. Then the ATT-AuNCs were directly immobilized on the test line of LFA strips as reporters. The sensing platform exhibited a linear response to melamine from 1 to 100 μM (R2 = 0.997) with a detection limit of 217 nM. Moreover, this LFA strip could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min. The recoveries of 91.6–101.5% were attained in milk and baby formula samples. This strip inherited the superior characteristics of low background interference of aggregation-induced fluorescence recognition and LFA technology, thus effectively avoiding the use of antibodies, aptamers, and other biological elements.</description><subject>Aggregation induced emission</subject><subject>Animals</subject><subject>detection limit</subject><subject>fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>food chemistry</subject><subject>Gold - chemistry</subject><subject>Gold nanocluster</subject><subject>Lateral flow assay</subject><subject>ligands</subject><subject>Limit of Detection</subject><subject>Melamine</subject><subject>Metal Nanoparticles - chemistry</subject><subject>milk</subject><subject>Milk - chemistry</subject><subject>nanogold</subject><subject>oligonucleotides</subject><subject>Spectrometry, Fluorescence</subject><subject>Triazines</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqNkU9v1DAQxS0EotvCV6h85JLt-E8S90ZVAa1UiQucrYk92XrlxFs7AfXb49W2XEE-2Hp6b2Y8P8YuBWwFiO5qvx1T8u6Rpq0EKbdCya6HN2wjTK-aHnr5lm1AgWmM0N0ZOy9lDwAShHnPzlSrhKlnw8oNL2E6xDAG8nyMa8pUHM0Lj7hQxli19JtjKfjMx5T5RBGnMBMfsNREmjnudpl2uIT6DrNfXZVpCqUchTTyXYqezzgnF9dSa5YP7N2IsdDHl_uC_fz65cftXfPw_dv97c1D4zSIpfFag3HKqL6Tg-laklogjFooQu3AtwMMon6iBzLe0ehbNNgLHAxey55adcE-neoecnpaqSy2TuUoRpwprcXKThvTSqHl_1ilktqo62rtTlaXUymZRnvIYcL8bAXYIxu7t69s7JGNPbGpwcuXHuswkf8be4VRDZ9PBqpL-RUo2-ICzXWfIZNbrE_hXz3-AMuxpH4</recordid><startdate>20220815</startdate><enddate>20220815</enddate><creator>Yue, Xin</creator><creator>Pan, Qiuli</creator><creator>Zhou, Jie</creator><creator>Ren, Hongxin</creator><creator>Peng, Chifang</creator><creator>Wang, Zhouping</creator><creator>Zhang, Yi</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20220815</creationdate><title>A simplified fluorescent lateral flow assay for melamine based on aggregation induced emission of gold nanoclusters</title><author>Yue, Xin ; Pan, Qiuli ; Zhou, Jie ; Ren, Hongxin ; Peng, Chifang ; Wang, Zhouping ; Zhang, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-d4408c383762b865e241a0f413ea4c0d5b0b118170e8dcefd5a8a71ab8a927e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aggregation induced emission</topic><topic>Animals</topic><topic>detection limit</topic><topic>fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>food chemistry</topic><topic>Gold - chemistry</topic><topic>Gold nanocluster</topic><topic>Lateral flow assay</topic><topic>ligands</topic><topic>Limit of Detection</topic><topic>Melamine</topic><topic>Metal Nanoparticles - chemistry</topic><topic>milk</topic><topic>Milk - chemistry</topic><topic>nanogold</topic><topic>oligonucleotides</topic><topic>Spectrometry, Fluorescence</topic><topic>Triazines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yue, Xin</creatorcontrib><creatorcontrib>Pan, Qiuli</creatorcontrib><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Ren, Hongxin</creatorcontrib><creatorcontrib>Peng, Chifang</creatorcontrib><creatorcontrib>Wang, Zhouping</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yue, Xin</au><au>Pan, Qiuli</au><au>Zhou, Jie</au><au>Ren, Hongxin</au><au>Peng, Chifang</au><au>Wang, Zhouping</au><au>Zhang, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simplified fluorescent lateral flow assay for melamine based on aggregation induced emission of gold nanoclusters</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2022-08-15</date><risdate>2022</risdate><volume>385</volume><spage>132670</spage><epage>132670</epage><pages>132670-132670</pages><artnum>132670</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>[Display omitted]
•A novel LFA was designed based on the AIE of AuNCs for the detection of melamine.•Molecular docking analysis verifies melamine has the highest affinity toward ATT.•The sensor could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min.
A novel lateral flow assay (LFA) was developed based on the aggregation induced emission (AIE) of gold nanoclusters (AuNCs) for the detection of melamine. We compared the interaction between melamine and AuNCs prepared with 6-Aza-2-thiothymine (ATT) and its four structural analogs as ligands and found that the ATT-AuNCs showed the best AIE induced by melamine. Then the ATT-AuNCs were directly immobilized on the test line of LFA strips as reporters. The sensing platform exhibited a linear response to melamine from 1 to 100 μM (R2 = 0.997) with a detection limit of 217 nM. Moreover, this LFA strip could discriminate 1.0 μM melamine clearly by the naked eyes within 3 min. The recoveries of 91.6–101.5% were attained in milk and baby formula samples. This strip inherited the superior characteristics of low background interference of aggregation-induced fluorescence recognition and LFA technology, thus effectively avoiding the use of antibodies, aptamers, and other biological elements.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>35318181</pmid><doi>10.1016/j.foodchem.2022.132670</doi><tpages>1</tpages></addata></record> |
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subjects | Aggregation induced emission Animals detection limit fluorescence Fluorescent Dyes - chemistry food chemistry Gold - chemistry Gold nanocluster Lateral flow assay ligands Limit of Detection Melamine Metal Nanoparticles - chemistry milk Milk - chemistry nanogold oligonucleotides Spectrometry, Fluorescence Triazines |
title | A simplified fluorescent lateral flow assay for melamine based on aggregation induced emission of gold nanoclusters |
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