Circ_0001093 promotes glutamine metabolism and cancer progression of esophageal squamous cell carcinoma by targeting miR-579-3p/glutaminase axis

• Published in: Journal of bioenergetics and biomembranes 2022-04, Vol.54 (2), p.119-134
• Main Authors: Qian, Cui-juan, Tong, Yi-yang, Wang, Yi-chao, Teng, Xiao-sheng, Yao, Jun
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Assaying; Biochemistry; Bioinformatics; Bioorganic Chemistry; Cancer; Cell growth; Cell Line, Tumor; Cell migration; Cell Movement; Cell proliferation; Chemistry; Chemistry and Materials Science; Cholecystokinin; Esophageal cancer; Esophageal Neoplasms - genetics; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma - genetics; Esophageal Squamous Cell Carcinoma - pathology; Esophagus; Gene Expression Regulation, Neoplastic; Glutaminase; Glutaminase - genetics; Glutaminase - metabolism; Glutamine; Glutamine - genetics; Glutamine - metabolism; Histology; Humans; Immunoprecipitation; Lymph nodes; Metabolism; Metastases; MicroRNAs - metabolism; miRNA; Morphology; mRNA; Organic Chemistry; Overexpression; Phenotypes; Ribonucleic acid; RNA; Squamous cell carcinoma; Tissues; Tumorigenesis; Tumors
• ISSN: 0145-479X; 1573-6881
• DOI: 10.1007/s10863-022-09935-6

Increasing studies indicate that circular RNAs (circRNAs) play critical roles in tumor metabolism of multiple cancers. However, the contribution of circRNAs in glutamine metabolism of esophageal squamous cell carcinoma (ESCC) remains elusive. The objective of this research was to investigate the role and mechanism of circRNA hsa_circ_0001093 (circ_0001093) in the glutamine metabolism and tumorigenesis of ESCC. Circ_0001093, microRNA-579-3p (miR-579-3p) and glutaminase (GLS) expressions in ESCC tissues and cell lines were measured by qRT-PCR, tissue array or Western blot. Cell proliferation, invasion and migration were assessed by CCK-8 or transwell assays. Glutamine consumption, glutamate and ATP production were detected by indicated assay kits. The relationships between circ_0001093 and miR-579-3p or GLS mRNA were investigated by bioinformatics analysis, RNA pull-down, luciferase reporter and RNA immunoprecipitation (RIP) assays. Here, we found that circ_0001093 expression was up-regulated in ESCC tissues and cell lines. Increased circ_0001093 expression predicted an unfavourable prognosis, and was associated with the lymph node metastasis, TNM staging and tumor size in ESCC tissues. Circ_0001093 knockdown suppressed cell proliferation, invasion, migration and glutamine metabolism of ESCC cells, while circ_0001093 over-expression showed the opposite effects. Mechanistically, circ_0001093 acted as a competing endogenous RNA (ceRNA) by sponging miR-579-3p, thereby increasing GLS expression. Furthermore, the inhibitory effects of circ_0001093 knockdown on the invasion, migration and glutamine metabolism were partly rescued by miR-579-3p inhibition or GLS over-expression in ESCC cells. Additionally, miR-579-3p expression was down-regulated in ESCC tissues, while GLS expression was up-regulated. In conclusion, this study first provides evidence that the circ_0001093/miR-579-3p/GLS regulatory network can affect glutamine metabolism and malignant phenotype of ESCC, which can further impact ESCC progression.