Effect of omentin-1 on cancer stem cell surface markers and tumour-suppressive miRNA expression in a high-glucose environment associated with colorectal cancer

To investigate how omentin-1 impacts colorectal cancer stem cell surface markers and the expression levels of tumour-suppressive micro ribonucleic acid in a colorectal cancer-associated high-glucose environment. The study was conducted in the First Affiliated Hospital of Anhui Medical University，Anh...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the Pakistan Medical Association 2022-03, Vol.72 (3), p.430-435
Main Authors: Hou, Lele, Xu, Murong, Zhao, Xiaotong, Chen, Mingwei, Hui, Cancan, Ji, Hua, Deng, Datong
Format: Article
Language:English
Subjects:
Biomarkers
Cancer cells
Colonic Neoplasms - genetics
Colorectal cancer
Cytokines
Development and progression
Genetic aspects
Glucose - pharmacology
Health aspects
Humans
MicroRNA
MicroRNAs - genetics
Neoplastic Stem Cells
Physiological aspects
Risk factors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To investigate how omentin-1 impacts colorectal cancer stem cell surface markers and the expression levels of tumour-suppressive micro ribonucleic acid in a colorectal cancer-associated high-glucose environment. The study was conducted in the First Affiliated Hospital of Anhui Medical University，Anhui, China，from April 2018 to January 2019 and comprised cluster of differentiation133 and colorectal cancer stem cells from the SW480 cell line（the human colon adenocarcinoma cell line） obtained through immunomagnetic beads-based cell isolation. The colon cancer stem cells were divided into 6 groups: Z0 (control), Z1 (1ug/mL omentin-1), Z2 (2ug/mL omentin-1), G0 (5.0g/mL glucose), G1 (1ug/mL omentin-1 and 5.0g/mL glucose), and G2 (2ug/mL omentin-1 and 5.0 g/mL glucose). After 24 hours of intervention, quantitative polymerase chain reaction and western blot test were used for the detection of messenger ribonucleic acid and protein levels of stem cell surface markers. The colorectal cancer stem cells were divided into three groups: the control group, omentin group 1 (1ug/mL omentin-1) and omentin group 2 (2ug/mL omentin-1). After 24 hours of intervention, the expression of tumour suppressor micro ribonucleic acid was measured using quantitative polymerase chain reaction. Data was analysed using SPSS 23. Compared to the Z0 group, cluster of differentiation133 messenger ribonucleic acid expression reduced sharply in Z1 group (p
ISSN:0030-9982
DOI:10.47391/JPMA.817