Comprehensive profiling of conjugated fatty acid isomers and their lipid oxidation products by two-dimensional chiral RP×RP liquid chromatography hyphenated to UV- and SWATH-MS-detection

This research reports on the development of a comprehensive two-dimensional liquid chromatography (2D-LC) method hyphenated to inline DAD-UV and ESI-QTOF-MS/MS-detection for the separation of conjugated polyunsaturated fatty acid isomers and structurally related (saturated, unconjugated, oxidized) c...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2022-04, Vol.1202, p.339667-339667, Article 339667
Main Authors: Olfert, Matthias, Bäurer, Stefanie, Wolter, Marc, Buckenmaier, Stephan, Brito-de la Fuente, Edmundo, Lämmerhofer, Michael
Format: Article
Language:English
Subjects:
Chromatography, Liquid - methods
Data-independent acquisition
Fatty Acids
Food analysis
Isomerism
LC×LC
Lipidomics
Lipids
Oxylipins
Pharmaceutical analysis
Tandem Mass Spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This research reports on the development of a comprehensive two-dimensional liquid chromatography (2D-LC) method hyphenated to inline DAD-UV and ESI-QTOF-MS/MS-detection for the separation of conjugated polyunsaturated fatty acid isomers and structurally related (saturated, unconjugated, oxidized) compounds. In pharmaceutical lipid formulations conjugated fatty acids can be found as impurities, generated by oxidation of polyunsaturated fatty acids. Due to the structural complexity of resultant multi-component samples one dimensional liquid chromatography may be suboptimal for quality control and impurity profiling. The screened reversed-phase columns showed a lack of selectivity for the conjugated fatty acid isomers but the resolutions improved with the shape selectivity of the stationary phases (C18- 
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2022.339667