An Engineered Bacteria-Hybrid Microrobot with the Magnetothermal Bioswitch for Remotely Collective Perception and Imaging-Guided Cancer Treatment

Microrobots driven by multiple propelling forces hold great potential for noninvasively targeted delivery in the physiologic environment. However, the remotely collective perception and precise propelling in a low Reynold’s number bioenvironment remain the major challenges of microrobots to achieve...

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Bibliographic Details
Published in:ACS nano 2022-04, Vol.16 (4), p.6118-6133
Main Authors: Chen, Haotian, Li, Yingze, Wang, Yanjin, Ning, Peng, Shen, Yajing, Wei, Xueyan, Feng, Qishuai, Liu, Yali, Li, Zhenguang, Xu, Chang, Huang, Siyu, Deng, Cuijun, Wang, Ping, Cheng, Yu
Format: Article
Language:English
Subjects:
Bacteria
Humans
Hypoxia
Neoplasms - diagnostic imaging
Neoplasms - drug therapy
Perception
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Summary:Microrobots driven by multiple propelling forces hold great potential for noninvasively targeted delivery in the physiologic environment. However, the remotely collective perception and precise propelling in a low Reynold’s number bioenvironment remain the major challenges of microrobots to achieve desired therapeutic effects in vivo. Here, we reported a biohybrid microrobot that integrated with magnetic, thermal, and hypoxia sensitivities and an internal fluorescent protein as the dual reporter of thermal and positioning signals for targeted cancer treatment. There were three key elements in the microrobotic system, including the magnetic nanoparticle (MNP)-loaded probiotic Escherichia coli Nissle1917 (EcN@MNP) for spatially magnetic and hypoxia perception, a thermal-logic circuit engineered into the bacteria to control the biosynthesis of mCherry as the temperature and positioning reporter, and NDH-2 enzyme encoded in the EcN for enhanced anticancer therapy. According to the fluorescent-protein-based imaging feedback, the microrobot showed good thermal sensitivity and active targeting ability to the tumor area in a collective manner under the magnetic field. The cancer cell apoptosis was efficiently triggered in vitro and in vivo by the hybrid microrobot coupled with the effects of magnetothermal ablation and NDH-2-induced reactive oxygen species (ROS) damage. Our study demonstrates that the biohybrid EcN microrobot is an ideal platform to integrate the physical, biological, and chemical properties for collective perception and propelling in targeted cancer treatment.
ISSN:1936-0851
1936-086X
DOI:10.1021/acsnano.1c11601