Nitric Oxide-Releasing Tryptophan-Based Poly(ester urea)s Electrospun Composite Nanofiber Mats with Antibacterial and Antibiofilm Activities for Infected Wound Healing

• Published in: ACS applied materials & interfaces 2022-04, Vol.14 (14), p.15911-15926
• Main Authors: Li, Mengna, Qiu, Weiwang, Wang, Qian, Li, Na, Liu, Lu, Wang, Xueli, Yu, Jianyong, Li, Xiaoran, Li, Faxue, Wu, Dequn
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Biofilms; Biological and Medical Applications of Materials and Interfaces; Esters; Humans; Nanofibers - therapeutic use; Nitric Oxide - chemistry; Nitric Oxide - pharmacology; Tryptophan - pharmacology; Urea - chemistry; Urea - pharmacology; Wound Healing; Wound Infection - drug therapy
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.1c24131

Bacterial biofilms on wounds can lead to ongoing inflammation and delayed reepithelialization, which brings a heavy burden to the medical systems. Nitric oxide based treatment has attracted attention because it is a promising strategy to eliminate biofilms and heal infected wounds. Herein, a series of tryptophan-based poly­(ester urea)­s with good biodegradation and biocompatibility were developed for the preparation of composite mats by electrospinning. Furthermore, the mats were grafted with a nitric oxide donor (nitrosoglutathione, GSNO) to provide one type of NO loading cargo. The mats were found to have a prolonged NO release profile for 408 h with a maximum release of 1.0 μmol/L, which had a significant effect on killing bacteria and destructing biofilms. The designed mats were demonstrated to promote the growth of cells, regulate inflammatory factors, and significantly improve collagen deposition in the wound, eventually accelerating wound-size reduction. Thus, the studies presented herein provide insights into the production of NO-releasing wound dressings and support the application of full-thickness wound healing.