Aflagellar Epimastigote of Trypanosoma caninum: Biological and Ultrastructural Study of this Atypical Evolutionary Form

• Published in: Acta parasitologica 2022-06, Vol.67 (2), p.912-920
• Main Authors: Nascimento, Kátia Cristina Silva, Souza, Sandra Maria de Oliveira, Fagundes, Aline, Silva, Roger Magno Macedo, de Oliveira Junior, Francisco Odencio Rodrigues, Corte-Real, Suzana, da Silva Barros, Juliana Helena
• Format: Article
• Language: English
• Subjects: Animal Systematics/Taxonomy/Biogeography; Animals; Axenic Culture; Biomedical and Life Sciences; Biomedicine; Cell culture; Cell differentiation; Cell Line; Cultivation; Culture Media; Dogs; Ecology; Evolution; Germfree; Growth curves; Lipid bodies; Lipids; Macrophages; Macrophages - parasitology; Medical Microbiology; Microbiology; Original Paper; Parasites; Parasitology; Pure culture; Trypanosoma; Trypanosoma cruzi
• ISSN: 1230-2821; 1896-1851
• DOI: 10.1007/s11686-022-00540-6

Purpose Trypanosoma caninum exhibits atypical epimastigote forms under axenic conditions. This study aimed to analyze this evolutionary form under different cultivation conditions and provide more information about this evolutionary form. Methods We selected a T. caninum isolate with a high percentage of aflagellar epimastigote forms in axenic cultures. Two separate growth curves were generated for T. caninum cultured in Schneider axenic medium and co-cultured with the DH82 cell line, followed by analysis and quantification of evolutionary forms using bright field microscopy. In addition, ultrastructural analysis of T. caninum was performed under both cultivation conditions. Results The growth curves of T. caninum under axenic and co-cultivation conditions exhibited similar profiles. However, in the axenic culture, the number of parasites was three times higher at the peak of the exponential phase than in the co-culture. In contrast to that in the axenic culture, in which only the epimastigote forms were observed along the entire curve, during co-cultivation with the DH82 cell line, differentiation was observed for the trypomastigote and spheromastigote forms in low proportions. These results demonstrated that when cultured alone, the T. caninum isolate preserved the aflagellar epimastigote form, but in the presence of DH82 canine macrophages, they differentiated into evolutionary forms, particularly trypomastigote forms. Moreover, this study is the first to describe the presence of lipid bodies, structure described as the parasite's nutritional reserve, throughout the body of T. caninum . Conclusions These findings describe biological and ultrastructural aspects of epimastigote aflagellar and suggest that this evolutionary form may be involved in the biological cycle of T. caninum , still unknown.