Circular RNA VMA21 ameliorates IL-1β-engendered chondrocyte injury through the miR-495-3p/FBWX7 signaling axis

This study explored the function of circular RNA VMA21 (circVMA21) in osteoarthritis (OA). IL-1β inducement reduced the expression of circVMA21 in C28/I2 cells and human primary chondrocytes. Forced expression of circVMA21 heightened cell viability and attenuated cell apoptosis, accompanied by upreg...

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Published in:Clinical immunology (Orlando, Fla.) Fla.), 2022-05, Vol.238, p.108995-108995, Article 108995
Main Authors: Li, ZhiFu, Meng, DongDong, Liu, YongYi, Bi, FangGang, Tian, Ke, Xu, JianZhong, Sun, JianGuang, Gu, ChenXi, Li, Yu
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - genetics
Chondrocytes - metabolism
circVMA21
Extracellular matrix
FBWX7
Interleukin-1beta - metabolism
MicroRNAs - genetics
MicroRNAs - metabolism
miR-495-3p
Osteoarthritis
Osteoarthritis - genetics
Osteoarthritis - metabolism
Rats
RNA, Circular - genetics
Signal Transduction
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Summary:This study explored the function of circular RNA VMA21 (circVMA21) in osteoarthritis (OA). IL-1β inducement reduced the expression of circVMA21 in C28/I2 cells and human primary chondrocytes. Forced expression of circVMA21 heightened cell viability and attenuated cell apoptosis, accompanied by upregulation of Bcl-2, and downregulation of Bax and C-caspase-3 in C28/I2 cells in response to IL-1β exposure. CircVMA21 overexpression diminished the expression of MMP1 and MMP13, augmented the expression of COL2A1, and impeded the production of IL-6, TNF-α, prostaglandin E2 (PGE2) and NO. CircVMA21 served as a competitive endogenous RNA by sponging miR-495-3p. F-box and WD40 domain protein 7 (FBWX7) was identified as a target of miR-495-3p. The compensation experiments affirmed that circVMA21-mediated protective effects on IL-1β-irritated chondrocytes through the miR-495-3p/FBWX7 axis. The role of circVMA21 was also confirmed in an OA rat model. Collectively, these findings revealed a protective effect of circVMA21in OA by intercepting the miR-495-3p/FBWX7 crosstalk. •CircVMA21 was diminished in human chondrocytes challenged by IL-1β.•CircVMA21 protected chondrocytes against IL-1β-triggered apoptosis, inflammatory insult and cartilage degradation.•CircVMA21 functioned as a ceRNA by efficaciously sponging miR-495-3p thereby adjusting FBWX7 expression.•The miR-495-3p/FBWX7 axis participated in the protective roles of circVMA21 in IL-1β-engendered chondrocyte damage.
ISSN:1521-6616
1521-7035
DOI:10.1016/j.clim.2022.108995