Circular RNA VMA21 ameliorates IL-1β-engendered chondrocyte injury through the miR-495-3p/FBWX7 signaling axis

• Published in: Clinical immunology (Orlando, Fla.) Fla.), 2022-05, Vol.238, p.108995-108995, Article 108995
• Main Authors: Li, ZhiFu, Meng, DongDong, Liu, YongYi, Bi, FangGang, Tian, Ke, Xu, JianZhong, Sun, JianGuang, Gu, ChenXi, Li, Yu
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - genetics; Chondrocytes - metabolism; circVMA21; Extracellular matrix; FBWX7; Interleukin-1beta - metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; miR-495-3p; Osteoarthritis; Osteoarthritis - genetics; Osteoarthritis - metabolism; Rats; RNA, Circular - genetics; Signal Transduction
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1016/j.clim.2022.108995

This study explored the function of circular RNA VMA21 (circVMA21) in osteoarthritis (OA). IL-1β inducement reduced the expression of circVMA21 in C28/I2 cells and human primary chondrocytes. Forced expression of circVMA21 heightened cell viability and attenuated cell apoptosis, accompanied by upregulation of Bcl-2, and downregulation of Bax and C-caspase-3 in C28/I2 cells in response to IL-1β exposure. CircVMA21 overexpression diminished the expression of MMP1 and MMP13, augmented the expression of COL2A1, and impeded the production of IL-6, TNF-α, prostaglandin E2 (PGE2) and NO. CircVMA21 served as a competitive endogenous RNA by sponging miR-495-3p. F-box and WD40 domain protein 7 (FBWX7) was identified as a target of miR-495-3p. The compensation experiments affirmed that circVMA21-mediated protective effects on IL-1β-irritated chondrocytes through the miR-495-3p/FBWX7 axis. The role of circVMA21 was also confirmed in an OA rat model. Collectively, these findings revealed a protective effect of circVMA21in OA by intercepting the miR-495-3p/FBWX7 crosstalk. •CircVMA21 was diminished in human chondrocytes challenged by IL-1β.•CircVMA21 protected chondrocytes against IL-1β-triggered apoptosis, inflammatory insult and cartilage degradation.•CircVMA21 functioned as a ceRNA by efficaciously sponging miR-495-3p thereby adjusting FBWX7 expression.•The miR-495-3p/FBWX7 axis participated in the protective roles of circVMA21 in IL-1β-engendered chondrocyte damage.