Understanding the complexity of epimorphic regeneration in zebrafish caudal fin tissue: A transcriptomic and proteomic approach

• Published in: Genomics (San Diego, Calif.) Calif.), 2022-03, Vol.114 (2), p.110300-110300, Article 110300
• Main Authors: Banu, Sarena, Gaur, Namami, Nair, Sowmya, Ravikrishnan, Tanuja, Khan, Shahida, Mani, Sandhya, Bharathi, Swarna, Mandal, Komal, Kuram, Naga Anusha, Vuppaladadium, Sowmya, Ravi, Rowmika, Murthy, Ch. Lakshmi N., Quoseena, Mir, Babu, Nukala Sarath, Idris, Mohammed M.
• Format: Article
• Language: English
• Subjects: Animal Fins - metabolism; Animals; Caudal fin; cell death; cell movement; cell proliferation; cell viability; Danio rerio; death; fins; genomics; interleukins; morbidity; necrosis; neurotransmitters; protein arginine methyltransferases; Proteomics; Regeneration; Regeneration - genetics; solutes; Transcriptome; Transcriptomics; Zebrafish; Zebrafish - genetics
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2022.110300

The complex epimorphic regeneration of zebrafish caudal fin tissue is hasty and absolute. This study was executed to understand the role of various genes/proteins involved in the regeneration of zebrafish caudal fin tissue through differential transcriptomics and proteomics analysis. Based on our study 1408 genes and 661 proteins were found differentially regulated in the regenerating caudal fin tissue for having at least 1-log fold change. Interleukin, Solute carrier, Protein arginine methyltransferase, Homeobox, Neurotransmitter and several novel genes were found to be associated with regeneration for its differential regulation during the mechanism. Based on the network and pathway analysis the differentially regulated genes and proteins were found allied with activation of cell proliferation, cell viability, cell survival & cell movement and inactivation of organismal death, morbidity, necrosis, death of embryo & cell death. This study has mapped a detailed insight of the genes/proteins expression associated with the epimorphic regeneration more profoundly. •1408 genes and 661 proteins were found differentially expressed during zebrafish caudal fin regeneration.•Interleukin, SLC, PRMT, HOX, neurotransmitter gene family were found to be associated with regeneration.•Cell proliferation, viability, survival and death are found to be associated with epimorphic regeneration.