Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein

A conducting molecularly imprinted polymer (MIP) film was integrated with an extended-gate field-effect transistor (EG-FET) transducer to determine epitopes of matrix metalloproteinase-1 (MMP-1) protein biomarker of idiopathic pulmonary fibrosis (IPF) selectively. Most suitable epitopes for imprinti...

Full description

Saved in:
Bibliographic Details
Published in:Biosensors & bioelectronics 2022-07, Vol.208, p.114203-114203, Article 114203
Main Authors: Bartold, Katarzyna, Iskierko, Zofia, Borowicz, Pawel, Noworyta, Krzysztof, Lin, Chu-Yun, Kalecki, Jakub, Sharma, Piyush Sindhu, Lin, Hung-Yin, Kutner, Wlodzimierz
Format: Article
Language:English
Subjects:
Biosensing Techniques
BLAST
Chemosensor
EG-FET
Epitope imprinting
Epitopes
Extended-gate field-effect transistor
Idiopathic pulmonary fibrosis
IPF
Matrix Metalloproteinase 1
MIP
MMP-1 protein
Molecular Imprinting
Molecularly imprinted polymer
Molecularly Imprinted Polymers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A conducting molecularly imprinted polymer (MIP) film was integrated with an extended-gate field-effect transistor (EG-FET) transducer to determine epitopes of matrix metalloproteinase-1 (MMP-1) protein biomarker of idiopathic pulmonary fibrosis (IPF) selectively. Most suitable epitopes for imprinting were selected with Basic Local Alignment Search Tool software. From a pool of MMP-1 epitopes, the two, i.e., MIAHDFPGIGHK and HGYPKDIYSS, the relatively short ones, most promising for MMP-1 determination, were selected, mainly considering their advantageous outermost location in the protein molecule and stability against aggregation. MIPs templated with selected epitopes of the MMP-1 protein were successfully prepared by potentiodynamic electropolymerization and simultaneously deposited as thin films on electrodes. The chemosensors, constructed of MIP films integrated with EG-FET, proved useful in determining these epitopes even in a medium as complex as a control serum. The limit of detection for the MIAHDFPGIGHK and HGYPKDIYSS epitope was ∼60 and 20 nM, respectively. Moreover, the chemosensors selectively recognized whole MMP-1 protein in the 50–500 nM concentration range in buffered control serum samples. [Display omitted]
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2022.114203