Photobiomodulation Therapy Affects the Elastic Modulus, Cytoskeletal Rearrangement and Migration Capability of Human Osteosarcoma Cells

• Published in: Lasers in medical science 2022-09, Vol.37 (7), p.2855-2863
• Main Authors: Barati Shoorche, Amin, Mohammadkarim, Alireza, Jadidi, Majid, Bahraminasab, Marjan
• Format: Article
• Language: English
• Subjects: Atomic force microscopy; Biomarkers; Bone cancer; Cell growth; Cell migration; Cell proliferation; Cytoskeleton; Dentistry; Exposure; Fluorescence; Gene expression; Gene rearrangement; Irradiation; Laser beams; Lasers; Light therapy; Mechanical properties; Medicine; Medicine & Public Health; Modulus of elasticity; Optical Devices; Optics; Original Article; Osteosarcoma; Osteosarcoma cells; Photonics; Quantum Optics; Radiation; Radiation effects; Sarcoma; Stiffening; Stiffness; Therapy; Wavelengths; Wound healing
• ISSN: 1435-604X; 0268-8921; 1435-604X
• DOI: 10.1007/s10103-022-03554-8

Photobiomodulation (PBM) therapy utilizes low-power lasers to modulate the viability of living human cells and leads to changes in proliferation, differentiation, adhesion and gene expression, even though the rearrangement of cytoskeleton was not previously studied. The present study aims to evaluate the photobiological effects on the elastic behavior of human osteosarcoma cells (MG-63) and their morphological changes. Fluorescence staining, confocal imaging and atomic force microscopy (AFM) topography were performed to study the effects of PBM therapy with the exposure of 532 nm-25mW, 650 nm-3mW, 650 nm-150mW and 780 nm-70mW beams following the 5-min continuous irradiation. The area of each beam was 3.14cm 2 with a source–surface distance of 20 cm. Besides the cell proliferation assessment, the migratory potential of MG-63 was determined with the wound healing technique. The results indicated an increase in stiffness and shape index of radiation-induced cells 24 h after exposure along with the obvious F-actins changes. But, cell stiffening was not observed 72 h after 532 nm laser irradiation. Also, a decrease in the migration rate was seen in all of the groups after 72 h of irradiation except cells treated with 532 nm wavelength. However, 532 nm laser beams increase the migratory potential 24 h after exposure. Within 72 h after irradiation, the cell proliferation was only affected by applying 532 nm and 650 nm-150mW laser beams. It was concluded that applying photobiomodulation with wavelengths of 650 nm (at both utilized powers) and 780 nm alters the migration capability and provides a quantitative description of cytoskeletal changes. Moreover, membrane stiffening can be considered as the biological marker of PBM treatments.