Description and genome analysis of Luteimonas viscosa sp. nov., a novel bacterium isolated from soil of a sunflower field

• Published in: Antonie van Leeuwenhoek 2022-06, Vol.115 (6), p.749-760
• Main Authors: Chen, Ya, Zhang, Ying, Xin, Di, Luo, Xiaonan, Pang, Huancheng, Li, Yuyi, Zhang, Jianli
• Format: Article
• Language: English
• Subjects: Alkaline phosphatase; Amino acids; Biomedical and Life Sciences; Carbohydrates; Cardiolipin; Catalase; Deoxyribonucleic acid; DNA; Fatty acids; Gene sequencing; Genes; Genomes; Helianthus; Hybridization; Life Sciences; Lipids; Medical Microbiology; Microbiology; New species; Nucleotide sequence; Nucleotides; Original Paper; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phylogenetics; Phylogeny; Plant Sciences; Quinones; rRNA 16S; Sodium chloride; Soil bacteria; Soil microorganisms; Soil Science & Conservation; Sunflowers
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-022-01730-y

Strain XBU10 T was isolated from a soil sample of a sunflower plot in Inner Mongolia, China. The isolate was a Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, and its colonies were bright yellow in colour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XBU10 T belonged to the genus Luteimonas of the family Lysobacteraceae and was most closely related to Luteimonas panaciterrae Gsoil 068 T (97.8%), Luteimonas marina FR1330 T (97.6%), Luteimonas aquatica RIB1-20 T (97.4%) and Luteimonas huabeiensis HB2 T (97.2%). Growth occurred at 4–40 °C (optimum, 28–30 °C), with 0–5.0% (w/v) NaCl (optimum, 0.5%) and at pH 6.0–10.0 (optimum, pH 7.0 − 8.0). The chemotaxonomic characteristics of strain XBU10 T , which had Q-8 as its predominant quinone and iso-C 17:1 ω 9 c , iso-C 15:0 , iso-C 17:0 and iso-C 16:0 as its major fatty acids, were consistent with classification in the genus Luteimonas . The polar lipid profile of strain XBU10 T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminophospholipids and three unidentified polar lipids. The genome of strain XBU10 T was 4.17 Mbp with a G + C content of 69.9%. Its genome sequence showed genes encoding alkaline phosphatase and catalase. Protein-coding genes related to carbohydrate-active enzymes were also observed. Average nucleotide identity (ANI) values between XBU10 T and other species of the genus Luteimonas were found to be low (ANIm