Functional investigation of chimeric antigen receptor T cells targeting LMP1 antigen

This study aimed to create a type of CAR-T cells that targets LMP1 antigen and study its immunotherapeutic effect on LMP1-positive hematological malignancies. To generate LMP1 CAR-T cells, a plasmid expressing LMP1 CAR was created using molecular cloning technology, and T cells were infected with LM...

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Published in:Zhōnghuá xuèyèxué zázhì 2022-03, Vol.43 (3), p.229-234
Main Authors: He, H Z, Xing, Y Y, Zhang, Y, Xu, Y X, Tian, Z, Xing, H Y, Tang, K J, Rao, Q, Wang, J X, Wang, M
Format: Article
Language:Chinese
Subjects:
Cell Line, Tumor
Herpesvirus 4, Human
Humans
Lentivirus
Lymphoma - therapy
Receptors, Chimeric Antigen - genetics
T-Lymphocytes
Viral Matrix Proteins
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container_issue 3
container_start_page 229
container_title Zhōnghuá xuèyèxué zázhì
container_volume 43
creator He, H Z
Xing, Y Y
Zhang, Y
Xu, Y X
Tian, Z
Xing, H Y
Tang, K J
Rao, Q
Wang, J X
Wang, M
description This study aimed to create a type of CAR-T cells that targets LMP1 antigen and study its immunotherapeutic effect on LMP1-positive hematological malignancies. To generate LMP1 CAR-T cells, a plasmid expressing LMP1 CAR was created using molecular cloning technology, and T cells were infected with LMP1 CAR lentivirus. The effects of LMP1 CAR-T cells on specific cytotoxicity against LMP1-positive tumor cell lines infected with the EB virus had been confirmed. ① LMP1 protein expressing on EB virus-positive lymphoma cells surface was verified. ② The LMP1 CAR-expressing plasmid was created, and LMP1 CAR-T cells were obtained by infecting T cells with a lentivirus packaging system, with an infection efficiency of more than 80% . ③LMP1 CAR-T cells have a 4∶1 effect-to-target ratio in killing LMP1-positive lymphoma cells. The killing effect of LMP1 CAR-T cells on Raji cells was enhanced after 48 h of coculture, but there was no significant killing effect on Ramos, which are LMP1-negative lymphoma cells. ④After cocult
doi_str_mv 10.3760/cma.j.issn.0253-2727.2022.03.008
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subjects Cell Line, Tumor
Herpesvirus 4, Human
Humans
Lentivirus
Lymphoma - therapy
Receptors, Chimeric Antigen - genetics
T-Lymphocytes
Viral Matrix Proteins
title Functional investigation of chimeric antigen receptor T cells targeting LMP1 antigen
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