Loading…
Synthetic vascular graft with spatially distinct architecture for rapid biomimetic cell organisation in a perfusion bioreactor
Access to lab-grown fully functional blood vessels would provide an invaluable resource to vascular medicine. The complex architecture and cellular makeup of native vessels, however, makes this extremely challenging to reproduce . Bioreactor systems have helped advanced research in this area by repl...
Saved in:
Published in: | Biomedical materials (Bristol) 2022-05, Vol.17 (4), p.45001 |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43 |
---|---|
cites | cdi_FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43 |
container_end_page | |
container_issue | 4 |
container_start_page | 45001 |
container_title | Biomedical materials (Bristol) |
container_volume | 17 |
creator | Michael, Praveesuda L Yang, Nianji Moore, Matthew Santos, Miguel Lam, Yuen Ting Ward, Annabelle Hung, Jui Chien Tan, Richard P Wise, Steven G |
description | Access to lab-grown fully functional blood vessels would provide an invaluable resource to vascular medicine. The complex architecture and cellular makeup of native vessels, however, makes this extremely challenging to reproduce
. Bioreactor systems have helped advanced research in this area by replicating many of the physiological conditions necessary for full-scale tissue growth outside of the body. A key element underpinning these technologies are 3D vascular graft templates which serve as temporary scaffolds to direct cell growth into similar cellular architectures observed in native vessels. Grafts further engineered with appropriate physical cues to accommodate the multiple cell types that reside within native vessels may help improve the production efficiency and physiological accuracy of bioreactor-grown vessel substitutes. Here, we engineered two distinct scaffold architectures into an electrospun vascular graft aiming to encourage the spatial organisation of human vascular endothelial cells (hCAECs) in a continuous luminal monolayer, co-cultured with human fibroblasts (hFBs) populating the graft wall. Using an electrospun composite of polycaprolactone and gelatin, we evaluated physical parameters including fibre diameter, fibre alignment, and porosity, that best mimicked the spatial composition and growth of hCAECs and hFBs in native vessels. Upon identifying the optimal scaffold architectures for each cell type, we constructed a custom-designed mandrel that combined these distinct architectures into a single vascular graft during a single electrospinning processing run. When connected to a perfusion bioreactor system, the dual architecture graft spatially oriented hCAECs and hFBs into the graft wall and lumen, respectively, directly from circulation. This biomimetic cell organisation was consistent with positive graft remodelling with significant collagen deposition in the graft wall. These findings demonstrate the influence of architectural cues to direct cell growth within vascular graft templates and the future potential of these approaches to more accurately and efficiency produce blood vessel substitutes in bioreactor systems. |
doi_str_mv | 10.1088/1748-605X/ac66b2 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2649997076</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2649997076</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43</originalsourceid><addsrcrecordid>eNp1kD1v1TAUhi0EoqWwMyFvMHCpHcd2MqKKL6kSAyCxWSfHJ72ukjjYTtFd-O0k3HInmPyh532P_TD2XIo3UjTNpbR1szNCf78ENKarHrDz09XD076WZ-xJzrdC6Far9jE7U7qWyor6nP36cpjKnkpAfgcZlwESv0nQF_4zlD3PM5QAw3DgPuQSJiwcEu5DISxLIt7HxBPMwfMuxDGMf4qQhoHHdANTyGs8TjxMHPhMqV_ydlzZRIAlpqfsUQ9Dpmf36wX79v7d16uPu-vPHz5dvb3eoTJN2aHvpVBSNVKgbqAlSxa1Qd-iV01Fhki11lggWXnou6rRurNIiNR5A7W6YK-OvXOKPxbKxY0hb--EieKSXWXqtm2tsGZFxRHFFHNO1Ls5hRHSwUnhNutu0-o2xe5ofY28uG9fupH8KfBX8wq8PAIhzu42LmlaP-u6cVy7XO1ErYWQbvb9Sr7-B_nfyb8BM52dhg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2649997076</pqid></control><display><type>article</type><title>Synthetic vascular graft with spatially distinct architecture for rapid biomimetic cell organisation in a perfusion bioreactor</title><source>Institute of Physics</source><creator>Michael, Praveesuda L ; Yang, Nianji ; Moore, Matthew ; Santos, Miguel ; Lam, Yuen Ting ; Ward, Annabelle ; Hung, Jui Chien ; Tan, Richard P ; Wise, Steven G</creator><creatorcontrib>Michael, Praveesuda L ; Yang, Nianji ; Moore, Matthew ; Santos, Miguel ; Lam, Yuen Ting ; Ward, Annabelle ; Hung, Jui Chien ; Tan, Richard P ; Wise, Steven G</creatorcontrib><description>Access to lab-grown fully functional blood vessels would provide an invaluable resource to vascular medicine. The complex architecture and cellular makeup of native vessels, however, makes this extremely challenging to reproduce
. Bioreactor systems have helped advanced research in this area by replicating many of the physiological conditions necessary for full-scale tissue growth outside of the body. A key element underpinning these technologies are 3D vascular graft templates which serve as temporary scaffolds to direct cell growth into similar cellular architectures observed in native vessels. Grafts further engineered with appropriate physical cues to accommodate the multiple cell types that reside within native vessels may help improve the production efficiency and physiological accuracy of bioreactor-grown vessel substitutes. Here, we engineered two distinct scaffold architectures into an electrospun vascular graft aiming to encourage the spatial organisation of human vascular endothelial cells (hCAECs) in a continuous luminal monolayer, co-cultured with human fibroblasts (hFBs) populating the graft wall. Using an electrospun composite of polycaprolactone and gelatin, we evaluated physical parameters including fibre diameter, fibre alignment, and porosity, that best mimicked the spatial composition and growth of hCAECs and hFBs in native vessels. Upon identifying the optimal scaffold architectures for each cell type, we constructed a custom-designed mandrel that combined these distinct architectures into a single vascular graft during a single electrospinning processing run. When connected to a perfusion bioreactor system, the dual architecture graft spatially oriented hCAECs and hFBs into the graft wall and lumen, respectively, directly from circulation. This biomimetic cell organisation was consistent with positive graft remodelling with significant collagen deposition in the graft wall. These findings demonstrate the influence of architectural cues to direct cell growth within vascular graft templates and the future potential of these approaches to more accurately and efficiency produce blood vessel substitutes in bioreactor systems.</description><identifier>ISSN: 1748-6041</identifier><identifier>EISSN: 1748-605X</identifier><identifier>DOI: 10.1088/1748-605X/ac66b2</identifier><identifier>PMID: 35413704</identifier><identifier>CODEN: BMBUCS</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Biomimetics ; Bioreactors ; Blood Vessel Prosthesis ; electrospinning ; Endothelial Cells - physiology ; Humans ; nanotopography ; Perfusion ; perfusion bioreactor ; scaffold architecture ; Tissue Engineering ; Tissue Scaffolds ; vascular graft</subject><ispartof>Biomedical materials (Bristol), 2022-05, Vol.17 (4), p.45001</ispartof><rights>2022 IOP Publishing Ltd</rights><rights>2022 IOP Publishing Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43</citedby><cites>FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43</cites><orcidid>0000-0001-7964-819X ; 0000-0002-2819-5407 ; 0000-0002-2670-2882 ; 0000-0003-0536-6418</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35413704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Michael, Praveesuda L</creatorcontrib><creatorcontrib>Yang, Nianji</creatorcontrib><creatorcontrib>Moore, Matthew</creatorcontrib><creatorcontrib>Santos, Miguel</creatorcontrib><creatorcontrib>Lam, Yuen Ting</creatorcontrib><creatorcontrib>Ward, Annabelle</creatorcontrib><creatorcontrib>Hung, Jui Chien</creatorcontrib><creatorcontrib>Tan, Richard P</creatorcontrib><creatorcontrib>Wise, Steven G</creatorcontrib><title>Synthetic vascular graft with spatially distinct architecture for rapid biomimetic cell organisation in a perfusion bioreactor</title><title>Biomedical materials (Bristol)</title><addtitle>BMM</addtitle><addtitle>Biomed. Mater</addtitle><description>Access to lab-grown fully functional blood vessels would provide an invaluable resource to vascular medicine. The complex architecture and cellular makeup of native vessels, however, makes this extremely challenging to reproduce
. Bioreactor systems have helped advanced research in this area by replicating many of the physiological conditions necessary for full-scale tissue growth outside of the body. A key element underpinning these technologies are 3D vascular graft templates which serve as temporary scaffolds to direct cell growth into similar cellular architectures observed in native vessels. Grafts further engineered with appropriate physical cues to accommodate the multiple cell types that reside within native vessels may help improve the production efficiency and physiological accuracy of bioreactor-grown vessel substitutes. Here, we engineered two distinct scaffold architectures into an electrospun vascular graft aiming to encourage the spatial organisation of human vascular endothelial cells (hCAECs) in a continuous luminal monolayer, co-cultured with human fibroblasts (hFBs) populating the graft wall. Using an electrospun composite of polycaprolactone and gelatin, we evaluated physical parameters including fibre diameter, fibre alignment, and porosity, that best mimicked the spatial composition and growth of hCAECs and hFBs in native vessels. Upon identifying the optimal scaffold architectures for each cell type, we constructed a custom-designed mandrel that combined these distinct architectures into a single vascular graft during a single electrospinning processing run. When connected to a perfusion bioreactor system, the dual architecture graft spatially oriented hCAECs and hFBs into the graft wall and lumen, respectively, directly from circulation. This biomimetic cell organisation was consistent with positive graft remodelling with significant collagen deposition in the graft wall. These findings demonstrate the influence of architectural cues to direct cell growth within vascular graft templates and the future potential of these approaches to more accurately and efficiency produce blood vessel substitutes in bioreactor systems.</description><subject>Biomimetics</subject><subject>Bioreactors</subject><subject>Blood Vessel Prosthesis</subject><subject>electrospinning</subject><subject>Endothelial Cells - physiology</subject><subject>Humans</subject><subject>nanotopography</subject><subject>Perfusion</subject><subject>perfusion bioreactor</subject><subject>scaffold architecture</subject><subject>Tissue Engineering</subject><subject>Tissue Scaffolds</subject><subject>vascular graft</subject><issn>1748-6041</issn><issn>1748-605X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kD1v1TAUhi0EoqWwMyFvMHCpHcd2MqKKL6kSAyCxWSfHJ72ukjjYTtFd-O0k3HInmPyh532P_TD2XIo3UjTNpbR1szNCf78ENKarHrDz09XD076WZ-xJzrdC6Far9jE7U7qWyor6nP36cpjKnkpAfgcZlwESv0nQF_4zlD3PM5QAw3DgPuQSJiwcEu5DISxLIt7HxBPMwfMuxDGMf4qQhoHHdANTyGs8TjxMHPhMqV_ydlzZRIAlpqfsUQ9Dpmf36wX79v7d16uPu-vPHz5dvb3eoTJN2aHvpVBSNVKgbqAlSxa1Qd-iV01Fhki11lggWXnou6rRurNIiNR5A7W6YK-OvXOKPxbKxY0hb--EieKSXWXqtm2tsGZFxRHFFHNO1Ls5hRHSwUnhNutu0-o2xe5ofY28uG9fupH8KfBX8wq8PAIhzu42LmlaP-u6cVy7XO1ErYWQbvb9Sr7-B_nfyb8BM52dhg</recordid><startdate>20220503</startdate><enddate>20220503</enddate><creator>Michael, Praveesuda L</creator><creator>Yang, Nianji</creator><creator>Moore, Matthew</creator><creator>Santos, Miguel</creator><creator>Lam, Yuen Ting</creator><creator>Ward, Annabelle</creator><creator>Hung, Jui Chien</creator><creator>Tan, Richard P</creator><creator>Wise, Steven G</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7964-819X</orcidid><orcidid>https://orcid.org/0000-0002-2819-5407</orcidid><orcidid>https://orcid.org/0000-0002-2670-2882</orcidid><orcidid>https://orcid.org/0000-0003-0536-6418</orcidid></search><sort><creationdate>20220503</creationdate><title>Synthetic vascular graft with spatially distinct architecture for rapid biomimetic cell organisation in a perfusion bioreactor</title><author>Michael, Praveesuda L ; Yang, Nianji ; Moore, Matthew ; Santos, Miguel ; Lam, Yuen Ting ; Ward, Annabelle ; Hung, Jui Chien ; Tan, Richard P ; Wise, Steven G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biomimetics</topic><topic>Bioreactors</topic><topic>Blood Vessel Prosthesis</topic><topic>electrospinning</topic><topic>Endothelial Cells - physiology</topic><topic>Humans</topic><topic>nanotopography</topic><topic>Perfusion</topic><topic>perfusion bioreactor</topic><topic>scaffold architecture</topic><topic>Tissue Engineering</topic><topic>Tissue Scaffolds</topic><topic>vascular graft</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michael, Praveesuda L</creatorcontrib><creatorcontrib>Yang, Nianji</creatorcontrib><creatorcontrib>Moore, Matthew</creatorcontrib><creatorcontrib>Santos, Miguel</creatorcontrib><creatorcontrib>Lam, Yuen Ting</creatorcontrib><creatorcontrib>Ward, Annabelle</creatorcontrib><creatorcontrib>Hung, Jui Chien</creatorcontrib><creatorcontrib>Tan, Richard P</creatorcontrib><creatorcontrib>Wise, Steven G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical materials (Bristol)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michael, Praveesuda L</au><au>Yang, Nianji</au><au>Moore, Matthew</au><au>Santos, Miguel</au><au>Lam, Yuen Ting</au><au>Ward, Annabelle</au><au>Hung, Jui Chien</au><au>Tan, Richard P</au><au>Wise, Steven G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthetic vascular graft with spatially distinct architecture for rapid biomimetic cell organisation in a perfusion bioreactor</atitle><jtitle>Biomedical materials (Bristol)</jtitle><stitle>BMM</stitle><addtitle>Biomed. Mater</addtitle><date>2022-05-03</date><risdate>2022</risdate><volume>17</volume><issue>4</issue><spage>45001</spage><pages>45001-</pages><issn>1748-6041</issn><eissn>1748-605X</eissn><coden>BMBUCS</coden><abstract>Access to lab-grown fully functional blood vessels would provide an invaluable resource to vascular medicine. The complex architecture and cellular makeup of native vessels, however, makes this extremely challenging to reproduce
. Bioreactor systems have helped advanced research in this area by replicating many of the physiological conditions necessary for full-scale tissue growth outside of the body. A key element underpinning these technologies are 3D vascular graft templates which serve as temporary scaffolds to direct cell growth into similar cellular architectures observed in native vessels. Grafts further engineered with appropriate physical cues to accommodate the multiple cell types that reside within native vessels may help improve the production efficiency and physiological accuracy of bioreactor-grown vessel substitutes. Here, we engineered two distinct scaffold architectures into an electrospun vascular graft aiming to encourage the spatial organisation of human vascular endothelial cells (hCAECs) in a continuous luminal monolayer, co-cultured with human fibroblasts (hFBs) populating the graft wall. Using an electrospun composite of polycaprolactone and gelatin, we evaluated physical parameters including fibre diameter, fibre alignment, and porosity, that best mimicked the spatial composition and growth of hCAECs and hFBs in native vessels. Upon identifying the optimal scaffold architectures for each cell type, we constructed a custom-designed mandrel that combined these distinct architectures into a single vascular graft during a single electrospinning processing run. When connected to a perfusion bioreactor system, the dual architecture graft spatially oriented hCAECs and hFBs into the graft wall and lumen, respectively, directly from circulation. This biomimetic cell organisation was consistent with positive graft remodelling with significant collagen deposition in the graft wall. These findings demonstrate the influence of architectural cues to direct cell growth within vascular graft templates and the future potential of these approaches to more accurately and efficiency produce blood vessel substitutes in bioreactor systems.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>35413704</pmid><doi>10.1088/1748-605X/ac66b2</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-7964-819X</orcidid><orcidid>https://orcid.org/0000-0002-2819-5407</orcidid><orcidid>https://orcid.org/0000-0002-2670-2882</orcidid><orcidid>https://orcid.org/0000-0003-0536-6418</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1748-6041 |
ispartof | Biomedical materials (Bristol), 2022-05, Vol.17 (4), p.45001 |
issn | 1748-6041 1748-605X |
language | eng |
recordid | cdi_proquest_miscellaneous_2649997076 |
source | Institute of Physics |
subjects | Biomimetics Bioreactors Blood Vessel Prosthesis electrospinning Endothelial Cells - physiology Humans nanotopography Perfusion perfusion bioreactor scaffold architecture Tissue Engineering Tissue Scaffolds vascular graft |
title | Synthetic vascular graft with spatially distinct architecture for rapid biomimetic cell organisation in a perfusion bioreactor |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T11%3A04%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Synthetic%20vascular%20graft%20with%20spatially%20distinct%20architecture%20for%20rapid%20biomimetic%20cell%20organisation%20in%20a%20perfusion%20bioreactor&rft.jtitle=Biomedical%20materials%20(Bristol)&rft.au=Michael,%20Praveesuda%20L&rft.date=2022-05-03&rft.volume=17&rft.issue=4&rft.spage=45001&rft.pages=45001-&rft.issn=1748-6041&rft.eissn=1748-605X&rft.coden=BMBUCS&rft_id=info:doi/10.1088/1748-605X/ac66b2&rft_dat=%3Cproquest_cross%3E2649997076%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c368t-cdf10313810c58a9e7e7c56cd9cd382e6ee39767ae12dafb2855b7ceccebd6a43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2649997076&rft_id=info:pmid/35413704&rfr_iscdi=true |