Functional Specificity of Three α‑Arabinofuranosidases from Different Glycoside Hydrolase Families in Aspergillus niger An76

α-l-Arabinofuranosidase (Abf), a debranching enzyme that can remove arabinose substituents from arabinoxylan, promotes the hydrolysis of hemicellulose in plant biomass. However, the functional specificity of Abfs from different glycoside hydrolase (GH) families on the digestion of arabinoxylan and t...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2022-04, Vol.70 (16), p.5039-5048
Main Authors: Wu, Xiuyun, Zhang, Shu, Zhao, Sha, Dai, Lin, Huang, Shuxia, Liu, Xinli, Yu, Junhong, Wang, Lushan
Format: Article
Language:English
Subjects:
Arabinose - metabolism
Aspergillus niger - genetics
Aspergillus niger - metabolism
Biotechnology and Biological Transformations
Glycoside Hydrolases - chemistry
Humans
Hydrolysis
Substrate Specificity
Xylans - chemistry
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Summary:α-l-Arabinofuranosidase (Abf), a debranching enzyme that can remove arabinose substituents from arabinoxylan, promotes the hydrolysis of hemicellulose in plant biomass. However, the functional specificity of Abfs from different glycoside hydrolase (GH) families on the digestion of arabinoxylan and their synergistic interaction with xylanase have not been systematically studied. In this work, we characterized three Abfs (AxhA, AbfB, and AbfC) from GH62, GH54, and GH51 families in Aspergillus niger An76. Quantitative transcriptional analysis showed that expression of the axhA gene was upregulated as a result of induction by xylose substrates, whereas expression of the abfB gene was mainly induced by arabinose. Recombinant AxhA, AbfB, and AbfC exhibited different hydrolytic performances. AxhA showed the highest catalytic activity toward wheat arabinoxylan (WAX) and tended to hydrolyze monosubstituted arabinofuranose units, whereas AbfB had higher catalytic activity on AN and debranched arabinan (DAN), having the ability to cope with mono- and disubstituted arabinofuranose units. Furthermore, AbfC had greater arabinofuranosidase activity on p-nitrophenyl-α-l-arabinofuranoside (pNP-AraF) than on other substrates. Moreover, three Abfs displayed obvious synergistic action with GH11 xylanase XynB against WAX and barley husk residues. The elucidation of the degradation mechanisms of Abfs will lay a theoretical foundation for the efficient industrialized transformation of arabinoxylans.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.1c08388