Toxicological effects of tris(1,3-dichloro-2-propyl) phosphate in oyster Crassostrea gigas using proteomic and phosphoproteomic analyses

• Published in: Journal of hazardous materials 2022-07, Vol.434, p.128824-128824, Article 128824
• Main Authors: Yin, Chengcheng, Sun, Zuodeng, Ji, Chenglong, Li, Fei, Wu, Huifeng
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase; Animals; Crassostrea; Crassostrea gigas; Flame Retardants; Organophosphorus Compounds - toxicity; Phosphates - toxicity; Phosphoproteomics; Proteomics; Tris(1,3-dichloro-2-propyl) phosphate
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2022.128824

As a typical organophosphorus pollutant, tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) has been widely detected in aquatic environment. Previous studies showed that protein phosphorylation might be a vital way of TDCIPP to exert multiple toxic effects. However, there is a lack of high-throughput investigations on how TDCIPP affected protein phosphorylation. In this study, the toxicological effects of TDCIPP were explored by proteomic and phosphoproteomic analyses together with traditional means in oysters Crassostrea gigas treated with 0.5, 5 and 50 μg/L TDCIPP for 28 days. Integration of omic analyses revealed that TDCIPP dysregulated transcription, energy metabolism, and apoptosis and cell proliferation by either directly phosphorylating pivotal proteins or phosphorylating their upstream signaling pathways. The U-shaped response of acetylcholinesterase activities suggested the neurotoxicity of TDCIPP in a hormesis manner. What’s more, the increase in caspase-9 activity as well as the expression or phosphorylation alterations in eukaryotic translation initiation factor 4E, cell division control protein 42 and transforming growth factor-β1-induced protein indicated the disruption of homeostasis between apoptosis and cell proliferation, which was consistent with the observation of shedding of digestive cells. Overall, combination of proteomic and phosphoproteomic analyses showed the capability of identifying molecular events, which provided new insights into the toxicological mechanisms of TDCIPP. [Display omitted] •Integration of phosphoproteomics and proteomics revealed the toxic effects of TDCIPP.•TDCIPP induced neurotoxicity in a hormesis manner.•TDCIPP exerted toxicities by dysregulation of protein expression and phosphorylation.•TDCIPP disrupted signaling pathways by protein phosphorylation.•TDCIPP caused disruption of homeostasis between apoptosis and cell proliferation.