Target recognition triggered CRISPR-Cas12a assisted allosteric scaffold for sensitively analyzing bacterial infection after dental implantation

• Published in: Analytical biochemistry 2022-06, Vol.647, p.114666-114666, Article 114666
• Main Authors: Chen, Qing, Wen, Yongbin
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Infections - diagnosis; Bacterial Infections - genetics; CRISPR-Cas Systems; CRISPR-Cas12a; Dental Implantation; DNA; Humans; Limit of Detection; Rolling circle amplification
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/j.ab.2022.114666

Accurate identification and sensitive quantification of infected bacteria after dental implant both play crucial roles in early-diagnosis of bacterial infection and guiding medicine applications. Herein, we propose a sensitive and accurate bacteria detection method based on CRISPR-Cas12a system assisted allosteric scaffold. In the method, the allosteric scaffold takes the responsibility of specifically identifying target bacteria and inducing CRISPR-Cas12a based signal amplification. Eventually, the method exhibits a wide detection range from 6 × 106 cfu/mL to 6 × 102 cfu/mL with the limit of detection (LOD) of 47 cfu/mL. Furthermore, the established approach also possesses a high specificity due to high selectivity of aptamer and robust accuracy in recognizing double strand DNA by CRISPR-Cas12a system. We believe that this work can provide new strategies in the field of diagnosing bacterial infections after dental implantation. [Display omitted] •We propose a CRISPR-Cas12a assisted allosteric scaffold for sensitive analysis of bacteria.•The method exhibited a wide detection range from 6 × 106 cfu/mL to 6 × 102 cfu/mL with a the limit of detection of 47 cfu/mL.•Trans-cleavage activity of Cas12a enzyme endows the method a high sensitivity.