Surviving in the fish gut: Comparative inhibitory capacities against the host proteinases in cestodes of the genus Proteocephalus

Currently, little is known about inhibitory substances enabling tapeworms to settle in fish intestines thereby avoiding proteolysis. Contrary to previous studies with certain host–parasite pairs, this research compares the inhibitory capacities in three tapeworm species of the same genus Proteocepha...

Full description

Saved in:
Bibliographic Details
Published in:Journal of fish diseases 2022-07, Vol.45 (7), p.1011-1021
Main Authors: Izvekova, Galina I., Frolova, Tatyana V., Izvekov, Evgeny I., Zhokhov, Alexander E.
Format: Article
Language:English
Subjects:
Analytical methods
digestion
Electrophoresis
Endoparasites
Fish
Freshwater fishes
helminths
host–parasite relationship
Incubation period
Intestine
Intestines
Molecular weight
Mucosa
Parasites
proteinase inhibitors
Proteins
Proteolysis
Serine
Serine proteinase
Species
Survival
teleost fish
Trypsin
Worms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Currently, little is known about inhibitory substances enabling tapeworms to settle in fish intestines thereby avoiding proteolysis. Contrary to previous studies with certain host–parasite pairs, this research compares the inhibitory capacities in three tapeworm species of the same genus Proteocephalus from four different fishes (P. torulosus from dace and zope, P. sagittus from stone loach and P. cernuae from ruffe). The tapeworm extracts studied significantly reduced the activity of commercial trypsin (although to a lesser degree than the synthetic inhibitor of serine proteinases PMSF), displaying clear inter‐specific variation in worms’ inhibitory ability. We also measured the proteolytic activity of the host intestinal mucosa exposed to tapeworm extracts which served as inhibitors. Based on per cent inhibition values, all tapeworm extracts significantly suppressed the mucosal proteolytic activity, with marked differences between certain host–parasite pairs. SDS‐PAGE electrophoresis of the incubation media and extracts detected in each tapeworm species 20–36 protein bands with apparent molecular weights from 10–12 to 312.5 kDa, mostly below 50 kDa. The incubation medium and extract of each parasite shared one to six bands ranging from 12 to 35 kDa, depending on its species, with only four bands common for two or more species. The band profiles suggest that in various Proteocephalus species inhibitory capacities against host proteinases can be ensured by different proteins.
ISSN:0140-7775
1365-2761
DOI:10.1111/jfd.13624