Magnetic nickel nanostructure as cellulase immobilization surface for the hydrolysis of lignocellulosic biomass

In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stabi...

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Bibliographic Details
Published in:International journal of biological macromolecules 2022-06, Vol.209 (Pt A), p.1048-1053
Main Authors: Rashid, Shah Samiur, Mustafa, Abu Hasnat, Rahim, Mohd Hasbi Ab, Gunes, Burcu
Format: Article
Language:English
Subjects:
Amino functionalization
Biomass
Cellulase - chemistry
Cellulase immobilization
Enzymatic hydrolysis
Enzyme reusability
Enzyme Stability
Enzymes, Immobilized - chemistry
Hydrogen-Ion Concentration
Hydrolysis
Lignin
Magnetic Phenomena
Nanoparticles
Nickel
Nickel nanoparticles
Temperature
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Summary:In this research, a magnetic reusable nickel nanoparticle (NiNPs) supporting materials were prepared for cellulase enzyme immobilization. The immobilized cellulase showed high activity recovery, large & fast immobilization capacity and improved pH & temperature tolerance. The excellent stability and reusability enabled the immobilized cellulase to retain 84% of its initial activity after ten cycles. At 2 mg/mL enzyme concentration, highest 93% immobilization efficiency was achieved within two hours of immobilization. When the treatment temperature reached 40 °C and pH 5, the immobilized cellulase exhibited highest residual activity. The immobilized cellulase could be separated from the solution by a magnetic force. This study introduced a novel supporting material for cellulase immobilization, and the immobilized cellulase poses a great potential in the hydrolysis of lignocellulosic biomass which can used as an easily applicable and sustainable pre-treatment step for advanced biofuel production. [Display omitted]
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2022.04.072