Identification of Toxoplasma gondii adhesins through a machine learning approach

Toxoplasma gondii, as other apicomplexa, employs adhesins transmembrane proteins for binding and invasion to host cells. Search and characterization of adhesins is pivotal in understanding Apicomplexa invasion mechanisms and targeting new druggable candidates. This work developed a machine learning...

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Bibliographic Details
Published in:Experimental parasitology 2022-07, Vol.238, p.108261-108261, Article 108261
Main Authors: Valencia-Hernández, Juan D., Acosta-Dávila, John Alejandro, Arenas-García, Juan Camilo, García-López, Laura Lorena, Molina-Lara, Diego Alejandro, Arenas-Soto, Ailan Farid, Eraso-Ortiz, Diego A., Gómez-Marín, Jorge E.
Format: Article
Language:English
Subjects:
Co-precipitation assay
Machine learning
Structural modelling
Toxoplasma, surface proteins
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Summary:Toxoplasma gondii, as other apicomplexa, employs adhesins transmembrane proteins for binding and invasion to host cells. Search and characterization of adhesins is pivotal in understanding Apicomplexa invasion mechanisms and targeting new druggable candidates. This work developed a machine learning software called ApiPredictor UniQE V2.0, based on two approaches: support vector machines and multilayer perceptron, to predict adhesins proteins from amino acid sequences. By using ApiPredictor UniQE V2.0, five SAG-Related Sequences (SRSs) were identified within the Toxoplasma gondii proteome. One of those candidates, TgSRS12B, was cloned in plasmid pEXP5-CT/TOPO and expressed in E. coli BL21 DE3. The resulting recombinant protein was purified via affinity chromatography. Co-precipitation assays in CaCo and Muller cells showed interactions between TgSRS12B-His-tagged and the membrane fractions from both human cell lines. In conclusion, we demonstrated that ApiPredictor UniQE V2.0, a bioinformatic free software, was able to identify TgSRS12B as a new adhesin protein. Software on line API predictor. [Display omitted] •Machine learning software selection of adhesins.•Five candidates and one cloned, expressed and purified (SRS12B).•Coprecipitation assays with two human cell lines subcellular fractions identified interactors in membrane fractions.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2022.108261