Omega-3 polyunsaturated fatty acids: a modified approach for chemo-prevention of bladder cancer in a rat model and molecular studies of antineoplastic mechanisms

Objectives To test the chemo-preventative effects of omega-3 against bladder cancer (BC) induction in a rat model and its potential antineoplastic mechanisms. Material and methods Ninety male Fisher rats were divided into three groups during a 22-week protocol: group 1 (control), group 2 (Placebo + ...

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Bibliographic Details
Published in:Molecular biology reports 2022-07, Vol.49 (7), p.6357-6365
Main Authors: Fadallah, Mohamed, Zahran, Mohamed H., El-Assmy, Ahmed M., Barakat, Nashwa M., Khater, Sherry, Awadalla, Amira, Ahmed, Asmaa E., Ibrahiem, E. L. Housseiny I., Shokeir, Ahmed A.
Format: Article
Language:English
Subjects:
Angiogenesis
Animal Anatomy
Animal Biochemistry
Antioxidants
Biomedical and Life Sciences
Bladder cancer
Epigenetics
Gene expression
Histology
Inflammation
Interleukin 6
Life Sciences
Malignancy
miRNA
Morphology
Original Article
Oxidative stress
Polyunsaturated fatty acids
Rodents
Squamous cell carcinoma
Statistical analysis
Transitional cell carcinoma
Tumors
Vascular endothelial growth factor
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Summary:Objectives To test the chemo-preventative effects of omega-3 against bladder cancer (BC) induction in a rat model and its potential antineoplastic mechanisms. Material and methods Ninety male Fisher rats were divided into three groups during a 22-week protocol: group 1 (control), group 2 (Placebo + N-butyl-N-4- hydroxybutyl nitrosamine (BBN) for induction of BC and group 3 received omega-3 (1200 mg/kg/day) + BBN. At the end, blood samples and bladder tissues were collected and checked for the presence of malignancy, markers of angiogenesis (VEGF relative gene expression), inflammation (IL-6), proliferation (KI-67 expressions), oxidative stress (serum MDA and serum SOD) and epigenetic control (miRNA-145 level). Results At the end of the study, 60% and 86.6% rats survived in group 2 and 3 with significant weight loss among rats in group 2 when compared with other groups. In group 2, all rats developed visible bladder lesions of which five and 13 developed squamous cell carcinoma (SCC) and transitional cell carcinoma (TCC). In omega3-treated group, only one developed low grade SCC and one developed high grade non- invasive TCC. Bladders from omega-3-treated rats showed lower expression ofKI-67 ( p  
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-022-07445-7